Impact of O-acetylation on chitin oligosaccharides modulating inflammatory responses in LPS-induced RAW264.7 cells and mice

doi:10.1016/j.carres.2024.109177

IOCAS-IR > 实验海洋生物学重点实验室

	Impact of O-acetylation on chitin oligosaccharides modulating inflammatory responses in LPS-induced RAW264.7 cells and mice
	Ge, Xiangyun 1,2; Zhu, Siqi 2; Yang, Haoyue2 ; Wang, Xin 1,2; Li, Jingwen 2; Liu, Song 2,3; Xing, Ronge2,3 ; Li, Pengcheng2,3 ; Li, Kecheng 2,3
	2024-08-01
发表期刊	CARBOHYDRATE RESEARCH
ISSN	0008-6215
卷号	542 页码:11
通讯作者	Li, Kecheng(lkc@qdio.ac.cn)
摘要	Chitin oligosaccharides have garnered significant attention due to their biological activities, particularly their immunomodulatory properties. However, O-acetylation in chemically preparing chitin oligosaccharides seems inevitable and leads to some uncertainty on the bioactivity of chitin oligosaccharides. In this study, an O-acetylfree chitin oligosaccharides and three different O-acetylated chitin oligosaccharides with degree of polymerization ranging from 2 to 6 were prepared using ammonia hydrolysis, and their structures and detailed components were further characterized with FTIR, NMR and MS. Subsequently, the effects of O-acetylation on the immunomodulatory activity of chitin oligosaccharides were investigated in vitro and in vivo. The results suggested that the chitin oligosaccharides with O-acetylation exhibited better inflammatory inhibition than pure chitin oligosaccharides, significantly reducing the expression of inflammatory factors, such as IL-6 and iNOS, in the LPS-induced RAW264.7 macrophage. The chitin oligosaccharides with a degree of O-acetylation of 93 % was found to effectively alleviate LPS-induced endotoxemia in mice, including serum inflammation indices reduction and damage repairment of the intestinal liver, and kidney tissues.
关键词	Chitin oligosaccharides O-acetylation Immunomodulatory activity Endotoxemia
DOI	10.1016/j.carres.2024.109177
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[42276097] ; Natural Science Foundation of Shandong Province[ZR2022YQ37] ; Shandong Key RD Plan ; Major Scientific and Technological Innovation Project[2022CXGC020413] ; Project for Future Network from Chinese Academy of Sciences[058GJHZ2023044FN]
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
WOS类目	Biochemistry & Molecular Biology ; Chemistry, Applied ; Chemistry, Organic
WOS记录号	WOS:001273117000001
出版者	ELSEVIER SCI LTD
WOS关键词	CHITOSAN OLIGOSACCHARIDES ; OXIDATIVE STRESS ; MOLECULAR-WEIGHT ; DEACETYLATION ; GLUCOMANNAN ; POLYSACCHARIDE ; MACROPHAGES ; BIOACTIVITY ; PI3K/AKT
引用统计
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/186082
专题	实验海洋生物学重点实验室
通讯作者	Li, Kecheng
作者单位	1.Qingdao Agr Univ, Marine Sci & Engn Coll, Qingdao 266109, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 3.Qingdao Marine Sci & Technol Ctr, Lab Marine Drugs & Bioprod, Qingdao 266237, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Ge, Xiangyun,Zhu, Siqi,Yang, Haoyue,et al. Impact of O-acetylation on chitin oligosaccharides modulating inflammatory responses in LPS-induced RAW264.7 cells and mice[J]. CARBOHYDRATE RESEARCH,2024,542:11.
APA	Ge, Xiangyun.,Zhu, Siqi.,Yang, Haoyue.,Wang, Xin.,Li, Jingwen.,...&Li, Kecheng.(2024).Impact of O-acetylation on chitin oligosaccharides modulating inflammatory responses in LPS-induced RAW264.7 cells and mice.CARBOHYDRATE RESEARCH,542,11.
MLA	Ge, Xiangyun,et al."Impact of O-acetylation on chitin oligosaccharides modulating inflammatory responses in LPS-induced RAW264.7 cells and mice".CARBOHYDRATE RESEARCH 542(2024):11.