Horizontal gene transfer plays a crucial role in the development of antibiotic resistance in an antibiotic-free shrimp farming system

doi:10.1016/j.jhazmat.2024.135150

IOCAS-IR > 实验海洋生物学重点实验室

	Horizontal gene transfer plays a crucial role in the development of antibiotic resistance in an antibiotic-free shrimp farming system
	Chi, Wendan 1; Zou, Yan ; Qiu, Tianlong2 ; Shi, Wen 3; Tang, Liuqing 1; Xu, Mengxue 1; Wu, Haiyi 1,5; Luan, Xiao 4
	2024-09-05
发表期刊	JOURNAL OF HAZARDOUS MATERIALS
ISSN	0304-3894
卷号	476 页码:13
通讯作者	Wu, Haiyi(wuhaiyi1997@163.com) ; Luan, Xiao(luanxiao@iwhr.com)
摘要	Antibiotic selective pressure in aquaculture systems often results in the antibiotic resistance genes (ARGs) proliferation. Nonetheless, a paucity of data exists concerning the mechanisms of ARGs development in aquaculture systems without the influences of antibiotics. This study utilized metagenomic approaches to elucidate the dynamics and transfer mechanisms of ARGs throughout the aquaculture of Pacific white shrimp. A marked change in the resistome was observed throughout the aquaculture without antibiotics. The total ARGs relative abundance increased from 0.05 to 0.33 by day 90 of cultivation, with even higher in mixed wastewater (0.44). Both bacterial communities and mobile genetic elements play pivotal roles in the development of ARGs. Metagenomeassembled genomes showed enrichment of environmentally intrinsic ARGs on chromosomes including mac B and mdt K. The plasmid-mediated horizontal transfer was recognized as a principal factor contributing to the rise of ARGs, particularly for tet G and flo R, and this led to an escalation of resistance risk, peaking at a risks core of 35.43 on day 90. This study demonstrates that horizontal gene transfer plays a crucial role in ARGs development without antibiotic pressure, which can provide a theoretical foundation for controlling ARGs proliferation in aquaculture systems.
关键词	Aquaculture system Antibiotic resistance Horizontal gene transfer Risk assessment and control Bacterial community
DOI	10.1016/j.jhazmat.2024.135150
收录类别	SCI
语种	英语
资助项目	National Key Research and Development Program of China[2023YFC3207902] ; Key Research and Development Program of Shandong Province, China[2022CXGC010401] ; Key Research and Development Program of Shandong Province, China[2023CXGC010412] ; Earmarked fund for Modern Agricuture Shrimps and Crabs Industry Technology System of Shandong province[SDAIT-13-04]
WOS研究方向	Engineering ; Environmental Sciences & Ecology
WOS类目	Engineering, Environmental ; Environmental Sciences
WOS记录号	WOS:001268387000001
出版者	ELSEVIER
WOS关键词	WASTE-WATER TREATMENT ; HEALTH-RISK ; BACTERIA ; TETRACYCLINE ; FISH ; PERSISTENCE ; SULFONAMIDE
引用统计
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/186231
专题	实验海洋生物学重点实验室
通讯作者	Wu, Haiyi; Luan, Xiao
作者单位	1.Marine Sci Res Inst Shandong Prov, Qingdao 266104, Peoples R China 2.Qingdao Key Lab Coastal Ecol Restorat & Secur, Qingdao, Shandong, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 4.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Environm Aquat Chem, Beijing 100085, Peoples R China 5.China Inst Water Resources & Hydropower Res, State Key Lab Simulat & Regulat Water Cycle River, Beijing 100048, Peoples R China
推荐引用方式 GB/T 7714	Chi, Wendan,Zou, Yan,Qiu, Tianlong,et al. Horizontal gene transfer plays a crucial role in the development of antibiotic resistance in an antibiotic-free shrimp farming system[J]. JOURNAL OF HAZARDOUS MATERIALS,2024,476:13.
APA	Chi, Wendan.,Zou, Yan.,Qiu, Tianlong.,Shi, Wen.,Tang, Liuqing.,...&Luan, Xiao.(2024).Horizontal gene transfer plays a crucial role in the development of antibiotic resistance in an antibiotic-free shrimp farming system.JOURNAL OF HAZARDOUS MATERIALS,476,13.
MLA	Chi, Wendan,et al."Horizontal gene transfer plays a crucial role in the development of antibiotic resistance in an antibiotic-free shrimp farming system".JOURNAL OF HAZARDOUS MATERIALS 476(2024):13.