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Characterization of 12 single nucleotide polymorphisms (SNPs) in Pacific abalone, Haliotis discus hannai
Qi, Hai-Gang1,2; Liu, Xiao1; Zhang, Guo-Fan1; Liu, X, Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
2008-09-01
发表期刊MOLECULAR ECOLOGY RESOURCES
ISSN1755-098X
卷号8期号:5页码:974-976
文章类型Article
摘要We report here for the first time 12 polymorphic single nucleotide polymorphisms (SNPs) in a commercially important gastropod, Pacific abalone (Haliotis discus hannai) that were identified by searching expressed sequence tag database. These SNP loci (seven nuclear and five mitochondrial SNPs) were polymorphic among 37 wild abalone individuals, based on a four-primer allele-specific polymerase chain reaction analysis. All loci had two alleles and the minor allele frequency ranged from 0.027 to 0.473. For the seven nuclear SNPs, the expected and observed heterozygosities ranged from 0.053 to 0.499 and from 0.054 to 0.811, respectively.; We report here for the first time 12 polymorphic single nucleotide polymorphisms (SNPs) in a commercially important gastropod, Pacific abalone (Haliotis discus hannai) that were identified by searching expressed sequence tag database. These SNP loci (seven nuclear and five mitochondrial SNPs) were polymorphic among 37 wild abalone individuals, based on a four-primer allele-specific polymerase chain reaction analysis. All loci had two alleles and the minor allele frequency ranged from 0.027 to 0.473. For the seven nuclear SNPs, the expected and observed heterozygosities ranged from 0.053 to 0.499 and from 0.054 to 0.811, respectively.
关键词As-pcr Haliotis Discus Hannai Pacific Abalone Polymorphism Snp
学科领域Biochemistry & Molecular Biology ; Ecology ; Evolutionary Biology
DOI10.1111/j.1755-0998.2008.02118.x
URL查看原文
收录类别SCI
语种英语
WOS记录号WOS:000258997100008
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被引频次:10[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/1862
专题海洋生物技术研发中心
通讯作者Liu, X, Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
作者单位1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
第一作者单位中国科学院海洋研究所
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Qi, Hai-Gang,Liu, Xiao,Zhang, Guo-Fan,et al. Characterization of 12 single nucleotide polymorphisms (SNPs) in Pacific abalone, Haliotis discus hannai[J]. MOLECULAR ECOLOGY RESOURCES,2008,8(5):974-976.
APA Qi, Hai-Gang,Liu, Xiao,Zhang, Guo-Fan,&Liu, X, Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China.(2008).Characterization of 12 single nucleotide polymorphisms (SNPs) in Pacific abalone, Haliotis discus hannai.MOLECULAR ECOLOGY RESOURCES,8(5),974-976.
MLA Qi, Hai-Gang,et al."Characterization of 12 single nucleotide polymorphisms (SNPs) in Pacific abalone, Haliotis discus hannai".MOLECULAR ECOLOGY RESOURCES 8.5(2008):974-976.
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