CircRNA8388 functions as the sponge for miR-2392 during intestinal regeneration in sea cucumber Apostichopus japonicus

doi:10.1016/j.ijbiomac.2024.133302

	CircRNA8388 functions as the sponge for miR-2392 during intestinal regeneration in sea cucumber Apostichopus japonicus
	Su, Fang; Huo, Da ; Yang, Hongsheng; Sun, Lina 1
	2024-08-01
发表期刊	INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
ISSN	0141-8130
卷号	274 页码:7
通讯作者	Sun, Lina(sunlina@qdio.ac.cn)
摘要	The sea cucumber Apostichopus japonicus can expel internal organs under stress and regenerate them subsequently. However, growth is delayed during regeneration, significantly impacting the industry. Circular RNAs (circRNAs) are single-stranded circular RNA molecules produced through alternative splicing of mRNA precursors. They play crucial roles in regulating gene expression via the ceRNA mechanism. In this study, circRNA profiles of control and regenerated intestines were constructed. A total of 15,874 circRNAs were identified, with a length of 300-350 nucleotides (nt) being the most abundant. Sanger sequencing confirmed the circular structure of circRNA398. Compared with the normal intestine, 50 and 83 differentially expressed circRNAs (DEcircRNAs) were identified in the regenerated intestine at 1 and 3 days post evisceration (dpe), respectively. Gene ontology (GO) terms for signal transduction and development regulation were most significantly enriched in 1dpeVScon and 3dpeVScon treatments, respectively. The dual-luciferase assay revealed that circRNA8388 functions as a sponge for miR-2392, participating in the remodeling of the extracellular matrix (ECM). In conclusion, these findings will contribute to the enhancement of the non-coding RNA database for echinoderms and lay the groundwork for future investigations into circRNA regulation during intestinal regeneration.
关键词	Circular RNA Intestinal regeneration Apostichopus japonicus
DOI	10.1016/j.ijbiomac.2024.133302
收录类别	SCI
语种	英语
资助项目	Foundation of Shandong Province[202306279]
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry ; Polymer Science
WOS类目	Biochemistry & Molecular Biology ; Chemistry, Applied ; Polymer Science
WOS记录号	WOS:001262117300001
出版者	ELSEVIER
WOS关键词	CIRCULAR RNAS
引用统计
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/186396
专题	海洋生态与环境科学重点实验室实验海洋生物学重点实验室
通讯作者	Sun, Lina
作者单位	1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Expt Marine Biol, Qingdao 266071, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao 266237, Peoples R China 4.Qingdao Natl Lab Marine Sci & Technol, Marine Biol & Biotechnol, Qingdao 266237, Peoples R China 5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
通讯作者单位	海洋生态与环境科学重点实验室
推荐引用方式 GB/T 7714	Su, Fang,Huo, Da,Yang, Hongsheng,et al. CircRNA8388 functions as the sponge for miR-2392 during intestinal regeneration in sea cucumber Apostichopus japonicus[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2024,274:7.
APA	Su, Fang,Huo, Da,Yang, Hongsheng,&Sun, Lina.(2024).CircRNA8388 functions as the sponge for miR-2392 during intestinal regeneration in sea cucumber Apostichopus japonicus.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,274,7.
MLA	Su, Fang,et al."CircRNA8388 functions as the sponge for miR-2392 during intestinal regeneration in sea cucumber Apostichopus japonicus".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 274(2024):7.