Overexpression of MPV17/PMP22-like protein 2 gene decreases production of radical oxygen species in <i>Pyropia yezoensis</i> (Bangiales, Rhodophyta)

doi:10.1111/jpy.13474

IOCAS-IR > 实验海洋生物学重点实验室

	*Overexpression of MPV17/PMP22-like protein 2 gene decreases production of radical oxygen species in Pyropia yezoensis* (Bangiales, Rhodophyta)**
	Li, Yujie 1,2; Yang, Jiali1,2 ; Sun, Zhenjie 1; Niu, Jianfeng 1; Wang, Guangce 1,3,4
	2024-06-24
发表期刊	JOURNAL OF PHYCOLOGY
ISSN	0022-3646
页码	14
通讯作者	Niu, Jianfeng(jf_niu@qdio.ac.cn) ; Wang, Guangce(gcwang@qdio.ac.cn)
摘要	The northward shift of Pyropia yezoensis aquaculture required the breeding of germplasms with tolerance to the oxidative stress due to the high light conditions of the North Yellow Sea area. The MPV17/PMP22 family proteins were identified as a molecule related to reactive oxygen species (ROS) metabolism. Here, one of the MPV17 homolog genes designated as PyM-LP2 was selected for functional identification by introducing the encoding sequence region/reverse complementary fragment into the Py. yezoensis genome. Although the photosynthetic activity, the respiratory rate, and the ROS level in wild type (WT) and different gene-transformed algal strains showed similar levels under normal conditions, the overexpression (OE) strain exhibited higher values of photosynthesis, respiration, and reducing equivalents pool size but lower intracellular ROS production under stress conditions compared with the WT. Conversely, all the above parameters showed opposite variation trends in RNAi strain as those in the OE strain. This implied that the PyM-LP2 protein was involved in the mitigation of the oxidative stress. Sequence analysis revealed that this PyM-LP2 protein was assorted to peroxisomes and might serve as a poring channel for transferring malate (Mal) to peroxisomes. By overexpressing PyM-LP2, the transfer of Mal from chloroplasts to peroxisomes was enhanced under stress conditions, which promoted photorespiration and ultimately alleviated excessive reduction of the photosynthetic electron chain. This research lays the groundwork for the breeding of algae with enhanced resistance to oxidative stresses.
关键词	antioxidant capacity genetic breeding peroxisomal porin-like channel photorespiratory photosynthetic electron chain Pyropia yezoensis
DOI	10.1111/jpy.13474
收录类别	SCI
语种	英语
资助项目	Major Scientific and Technological Innovation Project of Shandong Provincial Key Research and Development Program[2022LZGC004] ; National Natural Science Foundation of China[42376091] ; China Agriculture Research System of MOF and MARA[CARS-50] ; Foundation of State Key Laboratory of Mariculture Biobreeding and Sustainable Goods[BRESG202308] ; Opening Foundation of Key Laboratory of Breeding Biotechnology and Sustainable Aquaculture, CAS[2023FB12] ; Research Fund for the Taishan Scholar Project of Shandong Province[2023FB12]
WOS研究方向	Plant Sciences ; Marine & Freshwater Biology
WOS类目	Plant Sciences ; Marine & Freshwater Biology
WOS记录号	WOS:001253940800001
出版者	WILEY
WOS关键词	PEROXISOMAL TARGETING SIGNAL ; SACCHAROMYCES-CEREVISIAE ; EXPRESSION ; MEMBRANE ; PHOTOSYNTHESIS ; CHLOROPLASTS ; RESPIRATION ; PROMOTER ; RECEPTOR ; STRESS
引用统计
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/186438
专题	实验海洋生物学重点实验室
通讯作者	Niu, Jianfeng; Wang, Guangce
作者单位	1.Chinese Acad Sci, Inst Oceanol, CAS & Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Qingdao Marine Sci & Technol Ctr, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 3.Chinese Acad Sci, Key Lab Breeding Biotechnol & Sustainable Aquacult, Qingdao, Peoples R China 4.Univ Chinese Acad Sci, Beijing, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Li, Yujie,Yang, Jiali,Sun, Zhenjie,et al. Overexpression of MPV17/PMP22-like protein 2 gene decreases production of radical oxygen species in Pyropia yezoensis (Bangiales, Rhodophyta)[J]. JOURNAL OF PHYCOLOGY,2024:14.
APA	Li, Yujie,Yang, Jiali,Sun, Zhenjie,Niu, Jianfeng,&Wang, Guangce.(2024).Overexpression of MPV17/PMP22-like protein 2 gene decreases production of radical oxygen species in Pyropia yezoensis (Bangiales, Rhodophyta).JOURNAL OF PHYCOLOGY,14.
MLA	Li, Yujie,et al."Overexpression of MPV17/PMP22-like protein 2 gene decreases production of radical oxygen species in Pyropia yezoensis (Bangiales, Rhodophyta)".JOURNAL OF PHYCOLOGY (2024):14.