Evaluating the effectiveness of the eRNA technique in monitoring fish biodiversity - A case study in the Qingdao offshore, China

doi:10.1016/j.gecco.2024.e02888

	Evaluating the effectiveness of the eRNA technique in monitoring fish biodiversity - A case study in the Qingdao offshore, China
	Li, Wenqiong1,2 ; Jia, Hui 1,3; Zhang, Hui1,2
	2024-06-01
发表期刊	GLOBAL ECOLOGY AND CONSERVATION
卷号	51 页码:17
通讯作者	Zhang, Hui(zhanghui@qdio.ac.cn)
摘要	With the continuous intensification of human activities, fish biodiversity has been reduced in the Yellow Sea. Therefore, accurate monitoring methods are crucial for determining fish biodiversity in the target area. The environmental DNA (eDNA) technique has emerged as a promising noninvasive method, but it is prone to false positives. The rapid degradation of environmental RNA (eRNA) has the potential to reduce false positives in the eDNA technique; however, there is limited research on using the eRNA technique to study fish biodiversity. This study aimed to explore a suitable sampling method for extracting sufficient eRNA from seawater samples. Furthermore, we performed a case study in Qingdao offshore, China, to survey fish species and community composition based on the eRNA and eDNA techniques in November 2022 using seawater samples collected from 19 sampling sites. We compared the results with data obtained through traditional field survey (TFS) methods and examined the relationship between fish species and environmental factors. The results revealed a significant overlap between fish species detected by the eRNA and eDNA techniques, with the eRNA technique demonstrating higher sensitivity and positive predictivity, and fewer false positives compared to those of eDNA. Our study highlighted the potential of the eRNA technique as a powerful tool for improving the detection rate of fish species in marine ecosystems.
关键词	eRNA technique eDNA technique Fish biodiversity Qingdao offshore
DOI	10.1016/j.gecco.2024.e02888
收录类别	SCI
语种	英语
资助项目	National Key Research and Development Project of China[2022YFE0112800] ; Taishan Scholars Program ; Youth Innovation Promotion Association CAS[2020211] ; Oceano-graphic Data Center, IOCAS
WOS研究方向	Biodiversity & Conservation ; Environmental Sciences & Ecology
WOS类目	Biodiversity Conservation ; Ecology
WOS记录号	WOS:001258492700001
出版者	ELSEVIER
WOS关键词	ENVIRONMENTAL DNA ; DEGRADATION ; DIVERSITY
引用统计
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/186468
专题	海洋生态与环境科学重点实验室
通讯作者	Zhang, Hui
作者单位	1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Ningbo Univ, Sch Marine Sci, Ningbo 315823, Peoples R China
第一作者单位	海洋生态与环境科学重点实验室
通讯作者单位	海洋生态与环境科学重点实验室
推荐引用方式 GB/T 7714	Li, Wenqiong,Jia, Hui,Zhang, Hui. Evaluating the effectiveness of the eRNA technique in monitoring fish biodiversity - A case study in the Qingdao offshore, China[J]. GLOBAL ECOLOGY AND CONSERVATION,2024,51:17.
APA	Li, Wenqiong,Jia, Hui,&Zhang, Hui.(2024).Evaluating the effectiveness of the eRNA technique in monitoring fish biodiversity - A case study in the Qingdao offshore, China.GLOBAL ECOLOGY AND CONSERVATION,51,17.
MLA	Li, Wenqiong,et al."Evaluating the effectiveness of the eRNA technique in monitoring fish biodiversity - A case study in the Qingdao offshore, China".GLOBAL ECOLOGY AND CONSERVATION 51(2024):17.