An antifouling electrochemical aptasensor based on a Y-shaped peptide for tetracycline detection in milk

doi:10.1016/j.jfca.2024.106349

IOCAS-IR

	An antifouling electrochemical aptasensor based on a Y-shaped peptide for tetracycline detection in milk
	Pu, Xujun 1; Hu, Yuanling 1; Niu, Meirong 1; Liu, Hongcheng 3; Li, Chenguo 2,4; Ma, Wenlong2 ; Gu, Ying 1
	2024-08-01
发表期刊	JOURNAL OF FOOD COMPOSITION AND ANALYSIS
ISSN	0889-1575
卷号	132 页码:10
通讯作者	Ma, Wenlong(mawenlong@qdio.ac.cn) ; Gu, Ying(guying@kust.edu.cn)
摘要	Nonspecific adsorption of non-target components (especially proteins) in food matrix on sensing surfaces deteriorates accuracy and sensitivity of electrochemical sensors, which is a key challenge for food safety sensing. Therefore, electrochemical sensors with antifouling capability of high-protein food matrices are highly desired. In this paper, an efficient and simple antifouling electrochemical aptasensor for tetracycline (TC) analysis was constructed based on a self-designed Y-shaped peptide [CPPPPEK-(RSERSERSE)2] consisting of cysteine as the anchoring segment, polyproline as the supporting segment, and -EK-(RSERSERSE)2 as antifouling branches. Hydrophilic and electrically neutral amino acid sequence and Y-shaped space structure of the peptide endow the sensing surface with good antifouling performance in protein solutions and diluted milk. Under optimized experiment conditions, the proposed aptasensor can detect TC with a wide linear range (0.01-100 ng mL-1) and a limit of detection of 5.3 pg mL-1 (S/N = 3). The spiked recovery experiments confirmed high accuracy of the aptasensor in diluted milk without other pretreatments (recoveries: 90.96%-95.86%). This strategy offers a valid way to reduce matrix interference, showing a promise to be extended to other sensing systems for different targets.
关键词	Antifouling Peptide Electrochemical aptasensor Tetracycline Protein Food safety
DOI	10.1016/j.jfca.2024.106349
收录类别	SCI
语种	英语
资助项目	Yunnan Major Scientific and Tech- nological Projects[202202AG050009] ; National Natural Science Foundation of China[32102073] ; Yunnan Funda- mental Research Projects[202201AU070106] ; Yunnan Funda- mental Research Projects[202101BE070001-052] ; Special Project for High-level Scientific and Technological Talents and Innovation Teams of Yunnan Province[202405AS350005]
WOS研究方向	Chemistry ; Food Science & Technology
WOS类目	Chemistry, Applied ; Food Science & Technology
WOS记录号	WOS:001245029600001
出版者	ACADEMIC PRESS INC ELSEVIER SCIENCE
WOS关键词	ZWITTERIONIC PEPTIDE ; PERFORMANCE
引用统计	被引频次：1[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/186555
专题	中国科学院海洋研究所
通讯作者	Ma, Wenlong; Gu, Ying
作者单位	1.Kunming Univ Sci & Technol, Fac Food Sci & Engn, Kunming 650500, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 3.Yunnan Acad Agr Sci, Qual Stand & Testing Technol Res Inst, Kunming 650205, Peoples R China 4.China Univ Petr, Coll Mech & Elect Engn, Qingdao 266580, Peoples R China
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Pu, Xujun,Hu, Yuanling,Niu, Meirong,et al. An antifouling electrochemical aptasensor based on a Y-shaped peptide for tetracycline detection in milk[J]. JOURNAL OF FOOD COMPOSITION AND ANALYSIS,2024,132:10.
APA	Pu, Xujun.,Hu, Yuanling.,Niu, Meirong.,Liu, Hongcheng.,Li, Chenguo.,...&Gu, Ying.(2024).An antifouling electrochemical aptasensor based on a Y-shaped peptide for tetracycline detection in milk.JOURNAL OF FOOD COMPOSITION AND ANALYSIS,132,10.
MLA	Pu, Xujun,et al."An antifouling electrochemical aptasensor based on a Y-shaped peptide for tetracycline detection in milk".JOURNAL OF FOOD COMPOSITION AND ANALYSIS 132(2024):10.