Comparative transcriptome analysis provides a glance into the regulatory of insulin-like peptide 1 gene in the Pacific white shrimp <i>Litopenaeus vannamei</i>

doi:10.1016/j.aquaculture.2024.741126

IOCAS-IR > 实验海洋生物学重点实验室

	Comparative transcriptome analysis provides a glance into the regulatory of insulin-like peptide 1 gene in the Pacific white shrimp Litopenaeus vannamei
	Su, Manwen1,2,3 ; Zhang, Xiaojun 1,2,3; Zhang, Xiaoxi1,2 ; Yuan, Jianbo 1,2,3; Yang, Ming 4; Li, Fuhua 1,2,3
	2024-10-15
发表期刊	AQUACULTURE
ISSN	0044-8486
卷号	591 页码:12
通讯作者	Zhang, Xiaojun(xjzhang@qdio.ac.cn)
摘要	Insulin -like peptides (ILPs) have been reported to exert significant and diverse regulatory effects through the insulin signaling pathway in invertebrates. However, there is a large research gap in the functional analysis of ILP genes in crustaceans. In this study, we combined RNA interference (RNAi) technology with transcriptome sequencing to investigate the regulatory effects of the ILP1 gene in the Pacific white shrimp Litopenaeus vannamei . After two weeks of continuous RNAi of LvILP1 , the shrimp in the dsLvILP1 group exhibited reduced body length gain, weight gain, molting rate, and hemolymph glucose concentration compared to the control dsEGFP group, indicating that knockdown of LvILP1 slowed shrimp growth, molting, and metabolism. Comparative transcriptome analysis of three tissues revealed that knockdown of LvILP1 inhibited muscle growth and development by modulating the mTOR and MAPK signaling pathways, and prevented the renewal of epidermal tissue through the Hippo signaling pathway. Meanwhile, it also suppressed certain growth signals in the hepatopancreas, such as juvenile hormone (JH) synthesis and 20-hydroxyecdysteroid (20E) signal activation. Additionally, it promoted the conversion pathway of some sugars into amino acid metabolism instead of energy metabolism after glycolysis in the hepatopancreas. Interestingly, knockdown of LvILP1 also affected carbohydrate metabolism by regulating the intracellular and extracellular transport of glucose and trehalose, as well as regulating the expression levels of key metabolic genes in three shrimp tissues. This disruption led to a decrease in blood glucose levels and suppressed energy production, ultimately providing less energy for shrimp growth and molting. This study provides valuable insights into the underlying genetic mechanism through which ILP1 regulates glucose metabolism and growth in shrimp. It enhances our understanding of the structure and function of the insulin pathway in crustaceans and serves as an important reference for the identification of candidate genes in molecular genetic breeding of shrimp.
关键词	Insulin-like peptide 1 Comparative transcriptome analysis Growth Molting Metabolism
DOI	10.1016/j.aquaculture.2024.741126
收录类别	SCI
语种	英语
资助项目	National Natural Sciences Foundation of China[32273102] ; National Natural Sciences Foundation of China[31972782] ; National Key Research and Development Program of China[2022YFF1000304] ; National Key Research and Development Program of China[2018YFD0900103] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDA24030105] ; Taishan Scholars Program and Oceanographic Data Center, IOCAS
WOS研究方向	Fisheries ; Marine & Freshwater Biology
WOS类目	Fisheries ; Marine & Freshwater Biology
WOS记录号	WOS:001247836700001
出版者	ELSEVIER
WOS关键词	JUVENILE-HORMONE ; EXPRESSION ; GROWTH ; HEMOLYMPH ; GLUCOSE ; INPUT ; LEVEL ; HISAT ; CYCLE
引用统计
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/186567
专题	实验海洋生物学重点实验室
通讯作者	Zhang, Xiaojun
作者单位	1.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, CAS & Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Key Lab Breeding Biotechnol & Sustainable Aquacult, Wuhan 430072, Peoples R China 3.Univ Chinese Acad Sci, Coll Earth Sci, Beijing 100049, Peoples R China 4.Ocean & Fishery Technol Ctr Fuzhou, Fuzhou 350005, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Su, Manwen,Zhang, Xiaojun,Zhang, Xiaoxi,et al. Comparative transcriptome analysis provides a glance into the regulatory of insulin-like peptide 1 gene in the Pacific white shrimp Litopenaeus vannamei[J]. AQUACULTURE,2024,591:12.
APA	Su, Manwen,Zhang, Xiaojun,Zhang, Xiaoxi,Yuan, Jianbo,Yang, Ming,&Li, Fuhua.(2024).Comparative transcriptome analysis provides a glance into the regulatory of insulin-like peptide 1 gene in the Pacific white shrimp Litopenaeus vannamei.AQUACULTURE,591,12.
MLA	Su, Manwen,et al."Comparative transcriptome analysis provides a glance into the regulatory of insulin-like peptide 1 gene in the Pacific white shrimp Litopenaeus vannamei".AQUACULTURE 591(2024):12.