Correlation of glutamate dehydrogenase gene polymorphism and mRNA expression level with free glutamate content in the clam Meretrix petechialis

doi:10.1016/j.aquaculture.2024.741130

IOCAS-IR > 实验海洋生物学重点实验室

	Correlation of glutamate dehydrogenase gene polymorphism and mRNA expression level with free glutamate content in the clam Meretrix petechialis
	Tian, Yanmin 1,4; Liu, Baozhong1,2,3 ; Yue, Xin 1; Wang, Hongxia 1
	2024-10-15
发表期刊	AQUACULTURE
ISSN	0044-8486
卷号	591 页码:9
通讯作者	Wang, Hongxia(hxwang@qdio.ac.cn)
摘要	Free glutamate is a characteristic amino acid with a fresh taste. A high free glutamate content can increase the flavour of clams and better meet consumer demands. GDH is involved in the production of glutamate from a-ketoglutarate and determines the speed and direction of reaction in the glutamate metabolic pathway. In this study, we cloned a full-length cDNA of MpGDH homologues in the clam Meretrix petechialis and further investigated the relationship among MpGDH gene polymorphisms, gene expression levels, enzyme activity and glutamate content traits. A significant positive correlation of MpGDH gene expression with free glutamate content was observed (r = 0.62, p < 0.05). The relationship of variation in MpGDH gene expression with its SNP genotype and SNP haplotype was assessed. Five SNPs were found to be significantly associated with MpGDH gene expression (p < 0.05), from which we constructed two main haplotypes due to linkage disequilibrium. The results showed that haplotype H2 may be an effective haplotype associated with high MpGDH gene expression and high free glutamate content. The genetic effect of different haplotypes was validated by different family materials, which showed that MpGDH gene expression level of the H1H2 heterozygous genotype were significantly higher than those of the homozygous genotype H1H1 (p < 0.05). In summary, our findings revealed the potential influence of the MpGDH polymorphism on the free glutamate content and provided molecular biological information for the selective breeding of good-quality traits of M. petechialis.
关键词	Meretrix petechialis Glutamate dehydrogenase Free glutamate content Gene polymorphism SNP
DOI	10.1016/j.aquaculture.2024.741130
收录类别	SCI
语种	英语
资助项目	Strategic Priority Research Program of the Chinese Academy of Sciences[XDA24030304] ; Key Research and Development Program of Shandong ; Taishan Industrial Leading Talents Project to Shandong Dehe Mingxing Biotechnology Co., Ltd. ; Natural Science Foundation of Shandong Province[ZR2020MD089] ; [CARS-49] ; [2021LZGC029]
WOS研究方向	Fisheries ; Marine & Freshwater Biology
WOS类目	Fisheries ; Marine & Freshwater Biology
WOS记录号	WOS:001247977900002
出版者	ELSEVIER
WOS关键词	MOLECULAR CHARACTERIZATION ; VIBRIO-RESISTANCE ; METABOLISM ; CHALLENGE ; GROWTH ; GDH
引用统计
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/186573
专题	实验海洋生物学重点实验室
通讯作者	Wang, Hongxia
作者单位	1.Chinese Acad Sci, Inst Oceanol, CAS & Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Qingdao Marine Sci & Technol Ctr, Lab Marine Biol & Biotechnol, Qingdao 266000, Peoples R China 3.Chinese Acad Sci, Key Lab Breeding Biotechnol & Sustainable Aquacult, Qingdao 266071, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Tian, Yanmin,Liu, Baozhong,Yue, Xin,et al. Correlation of glutamate dehydrogenase gene polymorphism and mRNA expression level with free glutamate content in the clam Meretrix petechialis[J]. AQUACULTURE,2024,591:9.
APA	Tian, Yanmin,Liu, Baozhong,Yue, Xin,&Wang, Hongxia.(2024).Correlation of glutamate dehydrogenase gene polymorphism and mRNA expression level with free glutamate content in the clam Meretrix petechialis.AQUACULTURE,591,9.
MLA	Tian, Yanmin,et al."Correlation of glutamate dehydrogenase gene polymorphism and mRNA expression level with free glutamate content in the clam Meretrix petechialis".AQUACULTURE 591(2024):9.