| Identification of the pathogenic ciliate Pseudocohnilembus persalinus (Oligohymenophorea: Scuticociliatia) by fluorescence in situ hybridization | |
Zhan, Zifeng1,2; Stoeck, Thorsten2; Dunthorn, Micah2; Xu, Kuidong1 ; Xu, KD (reprint author), Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China.
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| 2014-02-01 | |
| 发表期刊 | EUROPEAN JOURNAL OF PROTISTOLOGY
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| 卷号 | 50期号:1页码:16-24 |
| 文章类型 | Article |
| 摘要 | Many scuticociliatid ciliates are regarded as devastating pathogens in aquaculture. Among these, Pseudocohnilembus persalinus is a facultative pathogen that often results in refractory diseases of mariculture fish. Although traditional silver staining methods have been successfully used to identify these ciliates, their identification is hampered by their small size and their morphological similarity to closely related species. We designed an alternative method of identification of P persalinus using an SSU-rDNA targeted oligonucleotide probe labeled with a fluorochrome, and optimized in a fluorescence in situ hybridization (FISH) protocol. The assay results in a clear identification by strong fluorescence signals from the oligonucleotide probe. The method can be used for quick and early detection of P. persalinus infections on host fish, as well as other susceptible organisms in aquiculture water. It may also be used in studies of the geographical distribution of this scuticociliate. (C) 2013 Elsevier GmbH. All rights reserved. |
| 关键词 | Fluorescence In Situ Hybridization Molecular Methods Oligonucleotide Probes Scuticociliates Ssu-rdna |
| 学科领域 | Microbiology |
| DOI | 10.1016/j.ejop.2013.09.004 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000331682300003 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/23775 |
| 专题 | 海洋生物分类与系统演化实验室 |
| 通讯作者 | Xu, KD (reprint author), Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China. |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Univ Kaiserslautern, Dept Ecol, D-67653 Kaiserslautern, Germany |
| 第一作者单位 | 中国科学院海洋研究所 |
| 推荐引用方式 GB/T 7714 | Zhan, Zifeng,Stoeck, Thorsten,Dunthorn, Micah,et al. Identification of the pathogenic ciliate Pseudocohnilembus persalinus (Oligohymenophorea: Scuticociliatia) by fluorescence in situ hybridization[J]. EUROPEAN JOURNAL OF PROTISTOLOGY,2014,50(1):16-24. |
| APA | Zhan, Zifeng,Stoeck, Thorsten,Dunthorn, Micah,Xu, Kuidong,&Xu, KD .(2014).Identification of the pathogenic ciliate Pseudocohnilembus persalinus (Oligohymenophorea: Scuticociliatia) by fluorescence in situ hybridization.EUROPEAN JOURNAL OF PROTISTOLOGY,50(1),16-24. |
| MLA | Zhan, Zifeng,et al."Identification of the pathogenic ciliate Pseudocohnilembus persalinus (Oligohymenophorea: Scuticociliatia) by fluorescence in situ hybridization".EUROPEAN JOURNAL OF PROTISTOLOGY 50.1(2014):16-24. |
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