Institutional Repository of Key Laboratory of Marine Ecology & Environmental Sciences, CAS
| Molecular cloning of heat shock protein 10 (Hsp10) and 60 (Hsp60) cDNAs and their expression analysis under thermal stress in the sea cucumber Apostichopus japonicus | |
Xu, Dongxue1,2 ; Sun, Lina1; Liu, Shilin1; Zhang, Libin1; Ru, Xiaoshang3; Zhao, Ye1,2; Yang, Hongsheng1; Yang, HS (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Marine Ecol & Environm Sci, Nanhai Rd 7, Qingdao 266071, Shandong, Peoples R China.
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| 2014-05-01 | |
| 发表期刊 | COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
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| 卷号 | 171页码:49-57 |
| 文章类型 | Article |
| 摘要 | Hsp10 and Hsp60 are important heat shock proteins (HSPs), which might be indispensable in the heat shock response and many other physiological processes. We obtained full-length cDNAs of genes hsp10 and hsp60 and classified their mRNA expression levels under thermal stress in the sea cucumber Apostichopus japonicus. Full-length hsp10 cDNA was 1528 bp containing a 5' untranslated region (UTR) of 83 bp, a 3' UTR of 1133 bp and an open reading frame (ORF) of 312 bp encoding 103 amino acid residues. Full-length hsp60 cDNA was 2560 bp containing a 118-bp 5' UTR, a 678-bp 3' UTR and a 1764-bp ORF encoding 586 amino acid residues. The deduced amino acid sequences of Hsp10 and Hsp60 shared the highest identity with sequences of the sea urchin Strongylocentrotus purpuratus, and phylogenetic trees showed that the evolution of Hsp10 and Hsp60 was almost in accord with the evolution of species. Further analysis bg real-time PCR showed that the expression of hsp10 and hsp60 mRNA was highly up-regulated at 26 degrees C compared with other three groups (20, 22, 24 degrees C), and their expression in the intestine was in a time-dependent manner at 26 degrees C. The results suggested that hsp10 and hsp60 were involved in the heat-shock response in the sea cucumber A. japonicus. (c) 2014 Elsevier Inc. All rights reserved. |
| 关键词 | Apostichopus Japonicus Hsp10 Hsp60 Heat Shock Mrna Expression |
| 学科领域 | Biochemistry & Molecular Biology ; Zoology |
| DOI | 10.1016/j.cbpb.2014.03.009 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000336704700007 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/24029 |
| 专题 | 海洋生态与环境科学重点实验室 |
| 通讯作者 | Yang, HS (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Marine Ecol & Environm Sci, Nanhai Rd 7, Qingdao 266071, Shandong, Peoples R China. |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Shandong, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Ocean Univ China, Coll Fisheries, Qingdao, Peoples R China |
| 第一作者单位 | 中国科学院海洋研究所 |
| 推荐引用方式 GB/T 7714 | Xu, Dongxue,Sun, Lina,Liu, Shilin,et al. Molecular cloning of heat shock protein 10 (Hsp10) and 60 (Hsp60) cDNAs and their expression analysis under thermal stress in the sea cucumber Apostichopus japonicus[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,2014,171:49-57. |
| APA | Xu, Dongxue.,Sun, Lina.,Liu, Shilin.,Zhang, Libin.,Ru, Xiaoshang.,...&Yang, HS .(2014).Molecular cloning of heat shock protein 10 (Hsp10) and 60 (Hsp60) cDNAs and their expression analysis under thermal stress in the sea cucumber Apostichopus japonicus.COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,171,49-57. |
| MLA | Xu, Dongxue,et al."Molecular cloning of heat shock protein 10 (Hsp10) and 60 (Hsp60) cDNAs and their expression analysis under thermal stress in the sea cucumber Apostichopus japonicus".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY 171(2014):49-57. |
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