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| Junctional adhesion molecule A of red drum (Sciaenops ocellatus): A possible immunomodulator and a target for bacterial immune evasion | |
| Zhang, Jian1,2; Zhang, Min1,3; Sun, Li1; Sun, L (reprint author), Chinese Acad Sci, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China. | |
| 2014-09-15 | |
| 发表期刊 | VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY
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| 卷号 | 161期号:1-2页码:99-107 |
| 文章类型 | Article |
| 摘要 | Junctional adhesion molecules (JAMs) are a family of type I cell surface receptors with two immunoglobulin (Ig) domains in the extracellular region. The family contains three classical members, i.e., JAM-A, -B, and -C. To date very little is known about the function of JAMs in teleost. In this work, we identified a JAM-A homologue (named SoJAMa) from red drum (Sciaenops ocellatus) and examined its expression and biological property. SoJAMa is composed of 347 amino acid residues and was predicted to be a transmembrane protein with a large extracellular region that contains two Ig domains. SoJAMa expression occurred in multiple tissues, in particular immune relevant organs. SoJAMa expression was down-regulated by experimental challenge with an extracellular pathogen but upregulated by challenge with an intracellular pathogen that is known to be capable of immune evasion. Likewise, cellular study showed that infection of peripheral blood leukocytes (PBL) with intracellular pathogen induced significantly higher expression of SoJAMa. Immunofluorescence microscopy showed that SoJAMa was localized on the surface of PBL and recognized by antibodies against recombinant SoJAMa. Blockage of the SoJAMa on PBL with antibodies resulted in augmented respiratory burst activity. Consistently, antibody-treated PBL exhibited enhanced resistance against bacterial infection. Taken together, these results suggest for the first time that a teleost JAM-A likely possesses immunoregulatory property in a negative manner, and that this property may be taken advantage of by intracellular pathogens as an invasion strategy. (C) 2014 Elsevier B.V. All rights reserved. |
| 关键词 | Junctional Adhesion Molecule Sciaenops Ocellatus Immunoregulation Intracellular Pathogen Immune Evasion |
| 学科领域 | Immunology ; Veterinary Sciences |
| DOI | 10.1016/j.vetimm.2014.07.008 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000342257400011 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/24125 |
| 专题 | 实验海洋生物学重点实验室 |
| 通讯作者 | Sun, L (reprint author), Chinese Acad Sci, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China. |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China 3.Qingdao Agr Univ, Coll Marine Sci & Engn, Qingdao 266109, Peoples R China |
| 第一作者单位 | 中国科学院海洋研究所 |
| 推荐引用方式 GB/T 7714 | Zhang, Jian,Zhang, Min,Sun, Li,et al. Junctional adhesion molecule A of red drum (Sciaenops ocellatus): A possible immunomodulator and a target for bacterial immune evasion[J]. VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY,2014,161(1-2):99-107. |
| APA | Zhang, Jian,Zhang, Min,Sun, Li,&Sun, L .(2014).Junctional adhesion molecule A of red drum (Sciaenops ocellatus): A possible immunomodulator and a target for bacterial immune evasion.VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY,161(1-2),99-107. |
| MLA | Zhang, Jian,et al."Junctional adhesion molecule A of red drum (Sciaenops ocellatus): A possible immunomodulator and a target for bacterial immune evasion".VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY 161.1-2(2014):99-107. |
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