Selection and Characterization of Aptamers Against Vibrio harveyi by SELEX

doi:10.3724/SP.J.1206.2013.00413

IOCAS-IR > 实验海洋生物学重点实验室

	Selection and Characterization of Aptamers Against Vibrio harveyi by SELEX
	Zheng Jiang 1,2; Hao Ju-Min 1; Song Lin-Sheng 2; Liu Rui 2; Song, LS (reprint author), Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China.
	2014-07-01
发表期刊	PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS
卷号	41 期号:7 页码:704-711
文章类型	Article
摘要	The disease caused by the opportunistic pathogen Vibrio harveyi is a serious infection which brings a heavy damage to aquaculture. Accurate and rapid detection of the microorganism is the first and necessary step to control the disease. Aptamers have a good potential application in the detection and identification of microorganisms because of their strong affinity, high specificity and good stability. In the present paper, aptamers targeting to V. harveyi were selected by the technique of systematic evolution of ligands by exponential enrichment (SELEX), and their affinities and specificities to V. harveyi were also evaluated. After 15 rounds of selection, the affinity of the aptamers in the enriched pool increased to 58.95, which was 16.8 times of that (3.51) in the original pool. After cloning and sequencing, 52 supposed aptamers were obtained from the enriched pool. These aptamers were divided into 8 families based on the homology analysis. Over 50% of these aptamers in the first and the second families shared higher homology, suggesting the selection was convergent and efficient. Six high-frequency aptamers were also found to have significant affinities and specificities to V. harveyi (P < 0.01). Five of them (S1, S25, S26, S27, S35) were proved to have higher affinities, and their affinity constants (K-d) were as followed: (32.6 +/- 7.1), (45.3 +/- 10.1), (24.7 +/- 5.8), (34.8 +/- 5.6) and (12.9 +/- 4.0) nmol/L, respectively. The production mechanism and the application values of high-frequency aptamers were also discussed. The present paper was the first report of aptamers with high affinity and specificity to the target V. harveyi, which would lay the foundation for the detection of the pathogenic microorganism based on aptamers.
关键词	Vibrio Harveyi Aptamer Affinity Affinity Constant (K-d) Selex
学科领域	Biochemistry & Molecular Biology ; Biophysics
DOI	10.3724/SP.J.1206.2013.00413
收录类别	SCI
语种	英语
WOS记录号	WOS:000339368200010
引用统计	被引频次：3[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/24162
专题	实验海洋生物学重点实验室
通讯作者	Song, LS (reprint author), Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China.
作者单位	1.Jimei Univ, Coll Fisheries, Key Lab Hlth Mariculture East China Sea, Minist Agr, Xiamen 361021, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Zheng Jiang,Hao Ju-Min,Song Lin-Sheng,et al. Selection and Characterization of Aptamers Against Vibrio harveyi by SELEX[J]. PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,2014,41(7):704-711.
APA	Zheng Jiang,Hao Ju-Min,Song Lin-Sheng,Liu Rui,&Song, LS .(2014).Selection and Characterization of Aptamers Against Vibrio harveyi by SELEX.PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,41(7),704-711.
MLA	Zheng Jiang,et al."Selection and Characterization of Aptamers Against Vibrio harveyi by SELEX".PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS 41.7(2014):704-711.

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