The immune responses triggered by CpG ODNs in shrimp Litopenaeus vannamei are associated with LvTolls

doi:10.1016/j.dci.2013.10.005

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	The immune responses triggered by CpG ODNs in shrimp Litopenaeus vannamei are associated with LvTolls
	Sun, Rui 1,2; Wang, Mengqiang1 ; Wang, Lingling 1; Yue, Feng 1,2; Yi, Qilin 1,2; Huang, Mengmeng1,2 ; Liu, Rui 1; Qiu, Limei 1; Song, Linsheng 1; Song, LS (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China.
	2014-03-01
发表期刊	DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
卷号	43 期号:1 页码:15-22
文章类型	Article
摘要	CpG oligodeoxynucleotides (ODNs) represent a kind of pathogen-associated molecular patterns (PAMPs) as well as a novel adjuvant that activate the innate immune system through interaction with Toll-like receptor 9 (TLR9) in mammals. In the present study, the synthetic oligodeoxynucleotides, CpG ODN 2395, was employed to investigate the interactive mode of CpG ODNs with three known Tolls (LvToll1-3) from shrimp Litopenaeus vannamei. The mature peptides of extracellular domains of LvTolls (LvToll-ECDs) were recombinant expressed and their binding activities to CpG ODN 2395 were further examined by ELISA. rLvToll1-ECD and rLvToll3-ECD exhibited affinity to CpG ODN 2395 in a dose-dependent manner when their concentrations ranged from 0.25 to 2.00 mu mol/L, while rLvToll2-ECD did not show any binding activities to CpG ODN 2395 in tested concentrations. Additionally, after the stimulation of CpG ODN 2395, the luciferase activities of HEK293T cells transfected with LvToll1-mosaic or LvToll3-mosaic were significantly increased to 2.38-fold (p < 0.01) and 1.56-fold (p < 0.01), while that in the HEK293T cells transfected with LvToll2-mosaic declined to 0.41-fold. The TNF-alpha activities were significantly enhanced (p < 0.01), and a significant increase (p < 0.05) of the NO production was observed at 12 h post CpG ODN 2395 stimulation. Moreover, the induced TNF-alpha activities and increased NO production triggered by CpG ODN 2395 were abolished after the treatment of chloroquine (CQ). The uptake of CpG ODN 2395 by shrimp haemocytes was investigated using the laser scanning confocal microscope, and CpG ODN 2395 was observed to be internalized by the haemocytes and distributed in the cytoplasm with aggregated signals around the nucleuses. It suggested that the interactions of CpG ODNs with LvToll1 and LvToll3 as well as the mature of endosomes in the haemocytes of shrimp L. vannamei were indispensable for the triggering of immune responses by CpG ODNs, and the results provided a foundation for the application of CpG ODNs as the novel immunostimulants in aquaculture. (C) 2013 Elsevier Ltd. All rights reserved.
关键词	Cpg Odns Litopenaeus Vannamei Toll Binding Activity Cellular Localization
学科领域	Immunology ; Zoology
DOI	10.1016/j.dci.2013.10.005
收录类别	SCI
语种	英语
WOS记录号	WOS:000331479800003
引用统计	被引频次：27[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/24177
专题	实验海洋生物学重点实验室
通讯作者	Song, LS (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China.
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
第一作者单位	实验海洋生物学重点实验室
推荐引用方式 GB/T 7714	Sun, Rui,Wang, Mengqiang,Wang, Lingling,et al. The immune responses triggered by CpG ODNs in shrimp Litopenaeus vannamei are associated with LvTolls[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2014,43(1):15-22.
APA	Sun, Rui.,Wang, Mengqiang.,Wang, Lingling.,Yue, Feng.,Yi, Qilin.,...&Song, LS .(2014).The immune responses triggered by CpG ODNs in shrimp Litopenaeus vannamei are associated with LvTolls.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,43(1),15-22.
MLA	Sun, Rui,et al."The immune responses triggered by CpG ODNs in shrimp Litopenaeus vannamei are associated with LvTolls".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 43.1(2014):15-22.

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