Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| Vibrio harveyi Hsp70: Immunogenicity and application in the development of an experimental vaccine against V. harveyi and Streptococcus iniae | |
Sun, Bo-guang1 ; Dang, Wei1,2,3; Sun, Li1; Hua, Yong-hua1; Hua, YH (reprint author), Chinese Acad Sci, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China.
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| 2014 | |
| 发表期刊 | AQUACULTURE
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| 卷号 | 418页码:144-147 |
| 文章类型 | Article |
| 摘要 | Vibrio harveyi and Streptococcus iniae are severe bacterial pathogens of farmed fish. In a previous study, we have identified a secretory antigen, Sia10, from S. iniae. In this study, we cloned and analyzed an hsp70 gene from a pathogenic V. harveyi strain. We found that purified recombinant Hsp70 possesses ATPase activity and, when used as a subunit vaccine, could induce protection in Japanese flounder (Paralichthys olivaceus) against V. harveyi infection. Based on this observation, we fused Hsp70 to Sia10 and thus obtained a chimeric antigen Sia10-Hsp70, which was expressed from a plasmid pTSH contained in Escherichia coli DH5 alpha. Western blot analysis showed that DH5 alpha/pTSH was able to secret Sia10-Hsp70 into the extracellular milieu. The potential of DH5 alpha/pTSH as a live vector vaccine was examined in a flounder model, which showed that DH5 alpha/pTSH induced effective protection against not only V. harveyi infection but also S. iniae infection. ELISA analysis showed that fish vaccinated with DH5 alpha/pTSH produced specific serum antibodies against both Hsp70 and Sia10. These results indicate that (i) V. harveyi Hsp70 is a biologically active protein with immunoprotective property; (ii) the chimeric antigen Sia10-Hsp70 delivered by a live bacterial host is an effective vaccine candidate against V. harveyi and S. iniae infection in aquaculture; (iii) using recombinant bacteria as vaccine hosts not only enables easier vaccine preparation but also easy construction of vaccines with cross protective potentials. (C) 2013 Elsevier B.V. All rights reserved. |
| 关键词 | Hsp70 Vibrio Harveyi Streptococcus Iniae Vaccine |
| 学科领域 | Fisheries ; Marine & Freshwater Biology |
| DOI | 10.1016/j.aquaculture.2013.10.018 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000326978300021 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/24192 |
| 专题 | 实验海洋生物学重点实验室 |
| 通讯作者 | Hua, YH (reprint author), Chinese Acad Sci, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China. |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Hangzhou Normal Univ, Coll Life & Environm Sci, Hangzhou Key Lab Anim Adaptat & Evolut, Hangzhou 310036, Zhejiang, Peoples R China 3.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
| 第一作者单位 | 实验海洋生物学重点实验室 |
| 推荐引用方式 GB/T 7714 | Sun, Bo-guang,Dang, Wei,Sun, Li,et al. Vibrio harveyi Hsp70: Immunogenicity and application in the development of an experimental vaccine against V. harveyi and Streptococcus iniae[J]. AQUACULTURE,2014,418:144-147. |
| APA | Sun, Bo-guang,Dang, Wei,Sun, Li,Hua, Yong-hua,&Hua, YH .(2014).Vibrio harveyi Hsp70: Immunogenicity and application in the development of an experimental vaccine against V. harveyi and Streptococcus iniae.AQUACULTURE,418,144-147. |
| MLA | Sun, Bo-guang,et al."Vibrio harveyi Hsp70: Immunogenicity and application in the development of an experimental vaccine against V. harveyi and Streptococcus iniae".AQUACULTURE 418(2014):144-147. |
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