Zoospore-derived monoecious gametophytes in Undaria pinnatifida (Phaeophyceae)

doi:10.1007/s00343-014-3139-x

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	Zoospore-derived monoecious gametophytes in Undaria pinnatifida (Phaeophyceae)
	Li Jing1,2 ; Pang Shaojun 1; Shan Tifeng 1; Liu Feng 1; Gao Suqin 1; Pang, SJ (reprint author), Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China.
	2014-03-01
发表期刊	CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
卷号	32 期号:2 页码:365-371
文章类型	Article
摘要	The annual life cycle of the brown seaweed Undaria pinnatifida (Harvey) Suringer comprises a macroscopic diploid sporophyte stage and a microscopic haploid gametophyte stage. In 2011, an unusual zoospore-derived monoecious gametophyte isolate (designated as line 10-5-3) of U. pinnatifida was observed. To understand this phenomenon, a comprehensive screening of eighty-two previously identified male gametophyte cultures, isolated from three randomly selected cultivars (lines 10, 7, and 5) was performed. Thirty-six of the isolates developed both antheridia and oogonia on the same filamentous fragment in a standard gametogenesis test (SGT: 18A degrees C, 60 mu mol photons/(m(2)center dot s)). Selfing of the monoecious gametophyte or crossing it with a normal male gametophyte both gave rise to morphologically normal sporophytic offspring. However, crossing resulted in a much higher fertilization rate (89.7%). The hybrid and selfed sporophytic offspring were grown to maturity in flow tanks at an ambient temperature of 10-18A degrees C over a period of 69 days. Active zoospores were released from both types of mature sporophylls. The majority of these developed into male gametophytes, while 15%-20% developed into the observed monoecious structures on the same filament. Using PCR amplification it was found that all the monoecious gametophyte isolates and the sporophytic offspring resulting from the selfing and crossing lacked the femalelinked microsatellite sequence (a part of the locus Up-AC-2A8, GenBank accession No. AY738602.1), indicating their male nature. U. pinnatifida is an invasive species in some regions and the implications of the above findings for this species in nature are briefly discussed.
关键词	Antheridia Kelp Monoecious Gametophyte Oogonia Undaria Pinnatifida
学科领域	Marine & Freshwater Biology ; Oceanography
DOI	10.1007/s00343-014-3139-x
收录类别	SCI
语种	英语
WOS记录号	WOS:000332739600013
引用统计	被引频次：14[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/24193
专题	实验海洋生物学重点实验室
通讯作者	Pang, SJ (reprint author), Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China.
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Li Jing,Pang Shaojun,Shan Tifeng,et al. Zoospore-derived monoecious gametophytes in Undaria pinnatifida (Phaeophyceae)[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2014,32(2):365-371.
APA	Li Jing,Pang Shaojun,Shan Tifeng,Liu Feng,Gao Suqin,&Pang, SJ .(2014).Zoospore-derived monoecious gametophytes in Undaria pinnatifida (Phaeophyceae).CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,32(2),365-371.
MLA	Li Jing,et al."Zoospore-derived monoecious gametophytes in Undaria pinnatifida (Phaeophyceae)".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 32.2(2014):365-371.

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