| Effect of dietary supplementation of vitamin C on growth, reactive oxygen species, and antioxidant enzyme activity of Apostichopus japonicus (Selenka) juveniles exposed to nitrite | |
| Luo Zuoyong1,2; Wang Baojie1; Liu Mei1; Jiang Keyong1; Liu Mingxing1; Wang Lei1; Wang, L (reprint author), Chinese Acad Sci, Inst Oceanol, Natl & Local Joint Engn Lab Ecol Mariculture, Qingdao 266071, Peoples R China. | |
| 2014-07-01 | |
| 发表期刊 | CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
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| 卷号 | 32期号:4页码:749-763 |
| 文章类型 | Article |
| 摘要 | Different amounts of vitamin C were added to diets fed to juveniles (2.5 +/- 0.15 g) of sea cucumber Apostichopus japonicus (Selenka) in an attempt to reduce the stress response of specimens exposed to nitrite stress. A commercial feed was used as the control diet and three experimental diets were made by supplementing 1 000, 1 500, or 2 000 mg vitamin C/kg diet to control diet separately in a 45-day experiment. Sea cucumbers were exposed to three different levels (0.5, 1.0, and 1.5 mg/L) of nitrite stress for 4, 8, and 12 h at four time intervals (0, 15, 30, and 45 d). Growth of the animals was recorded during the experiment. Reactive oxygen species (ROS) (i.e. hydroxyl free radical (-OH), malondialdehyde (MDA) and total antioxidant capacity (T-AOC)) and antioxidant enzyme activities (i. e., superoxide dismutase (SOD) and catalase (CAT)) were measured. Response surface methodology (RSM) was used to analyze the effect of multiple factors on ROS indices and enzyme activities. Weight gain (WG) and special growth rate (SGR) of vitamin C supplementation groups were significantly higher than those of control group (P<0.05). The levels of -OH and MDA increased under exposure time extending and nitrite concentration increasing, whereas T-AOC level decreased. SOD and CAT activities increased at 4 h and 8 h and decreased at 12 h. During the days in which the animal consumed experimental diets, the levels of -OH and MDA decreased and that of T-AOC increased. This result suggests that diets containing vitamin C could reduce the nitrite stress response in the animals and increase their antioxidant capacity. The multifactor regression equation of growth performance, ROS indices, and duration of feeding results suggest that vitamin C supplementation of 1 400-2 000 mg/kg diet for 29-35 days could reduce effectively the effects of nitrite exposure. |
| 关键词 | Sea Cucumber Apostichopus Japonicus Selenka Vitamin c Nitrite Stress Antioxidant Enzyme Reactive Oxygen Species (Ros) Response Surface Methodology (Rsm) |
| 学科领域 | Marine & Freshwater Biology ; Oceanography |
| DOI | 10.1007/s00343-014-3310-4 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000339046200003 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/24207 |
| 专题 | 海洋生物技术研发中心 |
| 通讯作者 | Wang, L (reprint author), Chinese Acad Sci, Inst Oceanol, Natl & Local Joint Engn Lab Ecol Mariculture, Qingdao 266071, Peoples R China. |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Natl & Local Joint Engn Lab Ecol Mariculture, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 第一作者单位 | 中国科学院海洋研究所 |
| 推荐引用方式 GB/T 7714 | Luo Zuoyong,Wang Baojie,Liu Mei,et al. Effect of dietary supplementation of vitamin C on growth, reactive oxygen species, and antioxidant enzyme activity of Apostichopus japonicus (Selenka) juveniles exposed to nitrite[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2014,32(4):749-763. |
| APA | Luo Zuoyong.,Wang Baojie.,Liu Mei.,Jiang Keyong.,Liu Mingxing.,...&Wang, L .(2014).Effect of dietary supplementation of vitamin C on growth, reactive oxygen species, and antioxidant enzyme activity of Apostichopus japonicus (Selenka) juveniles exposed to nitrite.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,32(4),749-763. |
| MLA | Luo Zuoyong,et al."Effect of dietary supplementation of vitamin C on growth, reactive oxygen species, and antioxidant enzyme activity of Apostichopus japonicus (Selenka) juveniles exposed to nitrite".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 32.4(2014):749-763. |
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