| Transcriptome Analysis Reveals a Rich Gene Set Related to Innate Immunity in the Eastern Oyster (Crassostrea virginica) | |
| Zhang, Linlin1,2; Li, Li1; Zhu, Yabing3; Zhang, Guofan1; Guo, Ximing4; Zhang, GF (reprint author), Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China. | |
| 2014-02-01 | |
| 发表期刊 | MARINE BIOTECHNOLOGY
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| 卷号 | 16期号:1页码:17-33 |
| 文章类型 | Article |
| 摘要 | As a benthic filter-feeder of estuaries, the eastern oyster, Crassostrea virginica, faces tremendous exposure to microbial pathogens. How eastern oysters without adaptive immunity survive in pathogen-rich environments is of fundamental interest, but studies on its immune system are hindered by the lack of genomic resources. We sequenced the transcriptome of an adult oyster with short Illumina reads and assembled 66,229 contigs with a N50 length of 1,503 bp. The assembly covered 89.4 % of published ESTs and 97.9 % of mitochondrial genes demonstrating its quality. A set of 39,978 contigs and unigenes (> 300 bp) were identified and annotated by searching public databases. Analysis of the gene set yielded a diverse set of 657 genes related to innate immunity, including many pertaining to pattern recognition, effectors, signal transduction, cytokines, and apoptosis. Gene families encoding C1q domain containing proteins, CTLD, IAPs, Ig_I-set, and TRAFs expanded in C. virginica and Crassostrea gigas. Many key genes of the apoptosis system including IAP, BAX, BAC-2, caspase, FADD, and TNFR were identified, suggesting C. virginica posses advanced apoptosis and apoptosis-regulating systems. Our results show that short Illumina reads can produce transcriptomes of highly polymorphic genomes with coverage and integrity comparable to that from longer 454 reads. The expansion and high diversity in gene families related to innate immunity, point to a complex defense system in the lophotrochozoan C. virginica, probably in adaptation to a pathogen-rich environment. |
| 关键词 | Innate Immunity Apoptosis Transcriptome Disease Adaptation Mollusca |
| 学科领域 | Biotechnology & Applied Microbiology ; Marine & Freshwater Biology |
| DOI | 10.1007/s10126-013-9526-z |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000330433700003 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/24249 |
| 专题 | 海洋生物技术研发中心 |
| 通讯作者 | Zhang, GF (reprint author), Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China. |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China 3.BGI Shenzhen, Shenzhen 518083, Peoples R China 4.Rutgers State Univ, Inst Marine & Coastal Sci, Haskin Shellfish Res Lab, Port Norris, NJ 08349 USA |
| 第一作者单位 | 中国科学院海洋研究所 |
| 推荐引用方式 GB/T 7714 | Zhang, Linlin,Li, Li,Zhu, Yabing,et al. Transcriptome Analysis Reveals a Rich Gene Set Related to Innate Immunity in the Eastern Oyster (Crassostrea virginica)[J]. MARINE BIOTECHNOLOGY,2014,16(1):17-33. |
| APA | Zhang, Linlin,Li, Li,Zhu, Yabing,Zhang, Guofan,Guo, Ximing,&Zhang, GF .(2014).Transcriptome Analysis Reveals a Rich Gene Set Related to Innate Immunity in the Eastern Oyster (Crassostrea virginica).MARINE BIOTECHNOLOGY,16(1),17-33. |
| MLA | Zhang, Linlin,et al."Transcriptome Analysis Reveals a Rich Gene Set Related to Innate Immunity in the Eastern Oyster (Crassostrea virginica)".MARINE BIOTECHNOLOGY 16.1(2014):17-33. |
| 条目包含的文件 | ||||||
| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| Transcriptome Analys（1259KB） | 限制开放 | ODC PDDL | 浏览 | |||
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