IOCAS-IR  > 海洋生物技术研发中心
Use of high-resolution melting analysis for detecting hybrids between the oysters Crassostrea sikamea and C-angulata reveals bidirectional gametic compatibility
Xu, Fei; Li, Li; Wang, Jiafeng; Zhang, Guofan; Zhang, GF (reprint author), Chinese Acad Sci, Inst Oceanol, Natl & Local Joint Engn Lab Ecol Mariculture, Qingdao 266071, Peoples R China.
2014-11-01
发表期刊JOURNAL OF MOLLUSCAN STUDIES
卷号80期号:4页码:435-443
文章类型Article
摘要To the best of our knowledge, the hybridization potential between the two closely related oyster species, Crassostrea sikamea and C. angulata, has yet to be reported. Moreover, hybrids obtained in most early experiments on oyster hybridization have been inadequately validated by genetic methods. In this study, a novel method based on high-resolution melting (HRM) analysis was used to visualize the coexistence of species-specific single nucleotide polymorphisms or insertion-deletion variations from both oyster species. Mitochondrial cytochrome c oxidase subunit I sequences were used to track the genetic material from a female to its descendants, while the nuclear genomic sequences of the first ribosomal internal transcribed spacer regions were used to track the genetic material from both parents and confirm hybrids of C. sikamea and C. angulata. Based on HRM analysis, bidirectional gametic compatibility between C. angulata and C. sikamea was successfully detected, although only several hundreds of larvae successfully hatched from tens of millions of C. angulata eggs inseminated by C. sikamea sperm, and the larvae were inviable. These results suggest the existence of bidirectional gametic compatibility between C. angulata and C. sikamea. Hybrids from C. sikamea eggs and C. angulata sperm showed higher growth rates and survival success during the swimming-larva stage, and may be potentially used for the genetic improvement of oyster aquaculture. This study provides a useful and reliable method for confirming hybrids in samples from either laboratory research or ecological field studies.
学科领域Marine & Freshwater Biology ; Zoology
DOI10.1093/mollus/eyu040
收录类别SCI
语种英语
WOS记录号WOS:000344623400010
引用统计
被引频次:14[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/24251
专题海洋生物技术研发中心
通讯作者Zhang, GF (reprint author), Chinese Acad Sci, Inst Oceanol, Natl & Local Joint Engn Lab Ecol Mariculture, Qingdao 266071, Peoples R China.
作者单位Chinese Acad Sci, Inst Oceanol, Natl & Local Joint Engn Lab Ecol Mariculture, Qingdao 266071, Peoples R China
第一作者单位中国科学院海洋研究所
推荐引用方式
GB/T 7714
Xu, Fei,Li, Li,Wang, Jiafeng,et al. Use of high-resolution melting analysis for detecting hybrids between the oysters Crassostrea sikamea and C-angulata reveals bidirectional gametic compatibility[J]. JOURNAL OF MOLLUSCAN STUDIES,2014,80(4):435-443.
APA Xu, Fei,Li, Li,Wang, Jiafeng,Zhang, Guofan,&Zhang, GF .(2014).Use of high-resolution melting analysis for detecting hybrids between the oysters Crassostrea sikamea and C-angulata reveals bidirectional gametic compatibility.JOURNAL OF MOLLUSCAN STUDIES,80(4),435-443.
MLA Xu, Fei,et al."Use of high-resolution melting analysis for detecting hybrids between the oysters Crassostrea sikamea and C-angulata reveals bidirectional gametic compatibility".JOURNAL OF MOLLUSCAN STUDIES 80.4(2014):435-443.
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
Use of high-resoluti(777KB) 限制开放ODC PDDL浏览
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Xu, Fei]的文章
[Li, Li]的文章
[Wang, Jiafeng]的文章
百度学术
百度学术中相似的文章
[Xu, Fei]的文章
[Li, Li]的文章
[Wang, Jiafeng]的文章
必应学术
必应学术中相似的文章
[Xu, Fei]的文章
[Li, Li]的文章
[Wang, Jiafeng]的文章
相关权益政策
暂无数据
收藏/分享
文件名: Use of high-resolution melting analysis for detecting hybrids between the oysters Crassostrea sikamea and C-angulata reveals bidirectional gametic compatibility.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。