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Comparative transcriptomic analysis provides insights into the molecular basis of the metamorphosis and nutrition metabolism change from zoeae to megalopae in Eriocheir sinensis
Li, Yingdong1; Hui, Min1; Cui, Zhaoxia1,2; Liu, Yuan1; Song, Chengwen1,3; Shi, Guohui1,3
2015-03-01
发表期刊COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS
卷号13页码:1-9
文章类型Article
摘要Within the larval period of Eriocheir sinensis, there is pronounced morphological changes upon the molt from the fifth zoeae (Z5) to megalopae (M), and low survival rate exists during this transition, which is typical in crab species. RNA sequencing was applied to 25 and M of E. sinensis, resulting in the discovery of 19,186 unigenes and 652 differentially expressed genes (DEGs, 3.40% of the unigenes). The important metabolic pathways that might play roles in the larval development of E sinensis from Z5 to M were detected to be 'Xenobiotics Biodegradation and Metabolism (8.16%)', 'Metabolism of Cofactors and Vitamins (6.70%)', 'Lipid Metabolism (6.36%)', and 'Amino Acid Metabolism (6.28%)'. Further, 19 DEGs possibly contributing to the morphological and sensory capability changes of the larvae were identified, like multiple copies of cuticle protein genes, retinaldehyde-binding protein 1 (RLBP1), envelope protein (Envelope) and hormone-related gene ecdysteroid-regulated 16 kDa protein (ESR16). Moreover, 62 DEGs were identified to be related to carbohydrate, lipid and protein digestion and metabolism, such as glucose dehydrogenases (GDHs), lipases (LIPS) and serine proteases (SPs). Among these DEGs, more genes related to the substance metabolism were found up-regulated in Z5 than M. suggesting that more energy might be essential to be released for 25 to complete the transition into M. Characterization of the crucial DEGs by real-time quantitative PCR re-conformed their expression pattern. This study provides the first genome-wide transcriptomic analysis of E. sinensis 25 and M for studying the molecular basis of the larvae metamorphosis and nutrition metabolism. (C) 2014 Elsevier Inc. All rights reserved.
关键词Larval Development Metamorphosis Mitten Crab Nutrition Metabolism Transcriptomics
DOI10.1016/j.cbd.2014.10.002
收录类别SCI
语种英语
WOS记录号WOS:000351032100001
引用统计
被引频次:38[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/25405
专题海洋生态与环境科学重点实验室
实验海洋生物学重点实验室
作者单位1.Chinese Acad Sci, Inst Oceanol, EMBL, Qingdao 266071, Peoples R China
2.Natl & Local Joint Engn Lab Ecol Mariculture, Qingdao 266071, Peoples R China
3.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
第一作者单位中国科学院海洋研究所
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Li, Yingdong,Hui, Min,Cui, Zhaoxia,et al. Comparative transcriptomic analysis provides insights into the molecular basis of the metamorphosis and nutrition metabolism change from zoeae to megalopae in Eriocheir sinensis[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS,2015,13:1-9.
APA Li, Yingdong,Hui, Min,Cui, Zhaoxia,Liu, Yuan,Song, Chengwen,&Shi, Guohui.(2015).Comparative transcriptomic analysis provides insights into the molecular basis of the metamorphosis and nutrition metabolism change from zoeae to megalopae in Eriocheir sinensis.COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS,13,1-9.
MLA Li, Yingdong,et al."Comparative transcriptomic analysis provides insights into the molecular basis of the metamorphosis and nutrition metabolism change from zoeae to megalopae in Eriocheir sinensis".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS 13(2015):1-9.
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