Molecular markers for identifying a new selected variety of Pacific white shrimp Litopenaeus vannamei

doi:10.1007/s00343-014-3232-1

IOCAS-IR > 实验海洋生物学重点实验室

	Molecular markers for identifying a new selected variety of Pacific white shrimp Litopenaeus vannamei
	Yu Yang1,2 ; Zhang Xiaojun 1; Liu Jingwen 1,2; Li Fuhua 1; Huang Hao 3; Li Yijun 3; Liu Xiaolin 4; Xiang Jianhai1
	2015
发表期刊	CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
卷号	33 期号:1 页码:1-10
文章类型	Article
摘要	Selective breeding of the Pacific white shrimp Litopenaeus vannamei during the last decade has produced new varieties exhibiting high growth rates and disease resistance. However, the identification of new varieties of shrimps from their phenotypic characters is difficult. This study introduces a new approach for identifying varieties of shrimps using molecular markers of microsatellites and mitochondrial control region sequences. The method was employed to identify a new selected variety, Kehai No. 1 (KH-1), from three representative stocks (control group): Zhengda; Tongwei; and a stock collected from Fujian Province, which is now cultured in mainland China. By pooled genotyping of KH-1 and the control group, five microsatellites showing differences between KH-1 and the control group were screened out. Individual genotyping data confirmed the results from pooled genotyping. The genotyping data for the five microsatellites were applied to the assignment analysis of the KH-1 group and the control group using the partial Bayesian assignment method in GENECLASS2. By sequencing the mitochondrial control regions of individuals from the KH-1 and control group, four haplotypes were observed in the KH-1 group, whereas 14 haplotypes were obtained in the control group. By combining the microsatellite assignment analysis with mitochondrial control region analysis, the average accuracy of identification of individuals in the KH-1 group and control group reached 89%. The five selected microsatellite loci and mitochondrial control region sequences were highly polymorphic and could be used to distinguish new selected varieties of L. vannamei from other populations cultured in China.
关键词	Molecular Identification Litopenaeus Vannamei New Variety Mitochondrial Control Region Microsatellite
DOI	10.1007/s00343-014-3232-1
收录类别	SCI
语种	英语
WOS记录号	WOS:000351156200001
引用统计	被引频次：7[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/25424
专题	实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Hainan Nanjiang Marine Biotech Co Ltd, Sanya 572536, Peoples R China 4.Northwest Agr & Forestry Univ, Yangling 712100, Peoples R China
第一作者单位	实验海洋生物学重点实验室
推荐引用方式 GB/T 7714	Yu Yang,Zhang Xiaojun,Liu Jingwen,et al. Molecular markers for identifying a new selected variety of Pacific white shrimp Litopenaeus vannamei[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2015,33(1):1-10.
APA	Yu Yang.,Zhang Xiaojun.,Liu Jingwen.,Li Fuhua.,Huang Hao.,...&Xiang Jianhai.(2015).Molecular markers for identifying a new selected variety of Pacific white shrimp Litopenaeus vannamei.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,33(1),1-10.
MLA	Yu Yang,et al."Molecular markers for identifying a new selected variety of Pacific white shrimp Litopenaeus vannamei".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 33.1(2015):1-10.

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