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Purification and characterization of novel antioxidant peptides of different molecular weights from mackerel Pneumatophorus japonicus protein hydrolysate
Wang Xueqin1,2; Xing Ronge1; Liu Song1; Yu Huahua1; Li Kecheng1; Chen Zuoyuan3; Li Pengcheng1
2015
发表期刊CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
卷号33期号:1页码:159-168
文章类型Article
摘要Mackerel (Pneumatophorus japonicus) proteins were hydrolyzed by five proteases: trypsin, papain, neutrase, acid protease, and flavourzyme. The hydrolysate treated by neutrase exhibited the highest antioxidant activity. Response surface methodology (RSM) was employed to optimize the hydrolysis conditions in an effort to obtain a mackerel protein hydrolysate (MPH) with the highest DPPH radical scavenging activity. The MPH was fractioned using a series of ultrafiltration membranes and five fractions, namely, MPH-I (>10 kDa), MPH-II (10-2.5 kDa), MPH-III (1-2.5 kDa), MPH-IV (0.4-1 kDa), and MPH-V (below 0.4 kDa), were obtained. DPPH radical scavenging activity, reducing power, hydroxyl radical scavenging activity, and the lipid peroxidation inhibition capability of these fractions were evaluated. The fractions in molecular weights <2.5 kDa (MPH-III, MPH-IV, and MPH-V), which occupied 93.4% of the total fractions, showed the strongest antioxidant activity; and the antioxidant activities of the three fractions are similar to each other. Using SP Sephadex C-25 and Sephadex G-25 columns, eight fractions were obtained from the MPH (<2.5 kDa). The isolated peptide I (1 664 kDa) displayed the highest DPPH radical scavenging activity. Therefore, MPH is a potential source of antioxidant peptides.
关键词Mackerel Protein Antioxidant Activity Fraction Of Hydrolysate Ultrafiltration Purification
DOI10.1007/s00343-014-3369-y
收录类别SCI
语种英语
WOS记录号WOS:000351156200019
引用统计
被引频次:7[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/25431
专题海洋生物技术研发中心
海洋环境工程技术研究发展中心
作者单位1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Qingdao Univ, Coll Med, Affiliated Hosp, Dept Cardiol, Qingdao 266003, Peoples R China
第一作者单位中国科学院海洋研究所
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Wang Xueqin,Xing Ronge,Liu Song,et al. Purification and characterization of novel antioxidant peptides of different molecular weights from mackerel Pneumatophorus japonicus protein hydrolysate[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2015,33(1):159-168.
APA Wang Xueqin.,Xing Ronge.,Liu Song.,Yu Huahua.,Li Kecheng.,...&Li Pengcheng.(2015).Purification and characterization of novel antioxidant peptides of different molecular weights from mackerel Pneumatophorus japonicus protein hydrolysate.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,33(1),159-168.
MLA Wang Xueqin,et al."Purification and characterization of novel antioxidant peptides of different molecular weights from mackerel Pneumatophorus japonicus protein hydrolysate".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 33.1(2015):159-168.
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