A single-CRD C-type lectin from oyster Crassostrea gigas mediates immune recognition and pathogen elimination with a potential role in the activation of complement system

doi:10.1016/j.fsi.2015.03.011

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	A single-CRD C-type lectin from oyster Crassostrea gigas mediates immune recognition and pathogen elimination with a potential role in the activation of complement system
	Li, Hui1,2 ; Zhang, Huan 1; Jiang, Shuai 1; Wang, Weilin1,2 ; Xin, Lusheng1,2 ; Wang, Hao 1; Wang, Lingling 1; Song, Linsheng 1,3
	2015-06-01
发表期刊	FISH & SHELLFISH IMMUNOLOGY
卷号	44 期号:2 页码:566-575
文章类型	Article
摘要	C-type lectins (CTLs), serving as pattern recognition receptors (PRRs), are a superfamily of Ca2+-dependent carbohydrate-recognition proteins that participate in nonself-recognition and pathogen elimination. In the present study, a single carbohydrate-recognition domain (CRD) CTL was identified from oyster Crassostrea gigas (designated as CgCLec-2). There was only one CRD within the deduced amino acid sequence of CgCLec-2 consisting of 129 amino acid residues. A conserved EPN (Glu(246)-Pro(247)-Asn(248)) motif was found in Ca2+-binding site 2 of CgCLec-2. The CgCLec-2 mRNA could be detected in all the examined tissues at different expression levels in oysters. The mRNA expression of CgCLec-2 in hemocytes was up-regulated significantly at 6 h post Vibrio splendidus challenge. The recombinant CgCLec-2 (rCgCLec-2) could bind various Pathogen-Associated Molecular Patterns (PAMPs), including lipopolysaccharide, mannan and peptidoglycan, and displayed strong binding abilities to Vibrio anguillarum, V. splendidus and Yarrowiali polytica and week binding ability to Staphylococcus aureus. It could also enhance the phagocytic activity of oyster hemocytes to V. splendidus and exhibited growth suppression activity against gram-positive bacteria S. aureus but no effect on gram-negative bacteria V. splendidus. Furthermore, the interaction between rCgCLec-2 and rCgMASPL-1 was confirmed by GST Pull down. The results suggested that CgCLec-2 served as not only a PRR in immune recognition but also a regulatory factor in pathogen elimination, and played a potential role in the activation of complement system. (c) 2015 Elsevier Ltd. All rights reserved.
关键词	Crassostrea Gigas C-type Lectin Innate Immunity Prr Opsonin Growth Suppression Activity Complement System
DOI	10.1016/j.fsi.2015.03.011
收录类别	SCI
语种	英语
WOS记录号	WOS:000354583800021
引用统计	被引频次：73[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/26971
专题	实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Dalian Ocean Univ, Dalian 116023, Peoples R China
第一作者单位	实验海洋生物学重点实验室
推荐引用方式 GB/T 7714	Li, Hui,Zhang, Huan,Jiang, Shuai,et al. A single-CRD C-type lectin from oyster Crassostrea gigas mediates immune recognition and pathogen elimination with a potential role in the activation of complement system[J]. FISH & SHELLFISH IMMUNOLOGY,2015,44(2):566-575.
APA	Li, Hui.,Zhang, Huan.,Jiang, Shuai.,Wang, Weilin.,Xin, Lusheng.,...&Song, Linsheng.(2015).A single-CRD C-type lectin from oyster Crassostrea gigas mediates immune recognition and pathogen elimination with a potential role in the activation of complement system.FISH & SHELLFISH IMMUNOLOGY,44(2),566-575.
MLA	Li, Hui,et al."A single-CRD C-type lectin from oyster Crassostrea gigas mediates immune recognition and pathogen elimination with a potential role in the activation of complement system".FISH & SHELLFISH IMMUNOLOGY 44.2(2015):566-575.

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