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Study on cytocompatibility of carboxymethyl-chitosan membranes to skin fibroblasts | |
Zheng, L; Chen, XG; Liu, WS; Han, XT; Yan, XJ | |
2003-04-01 | |
发表期刊 | PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS |
ISSN | 1000-3282 |
卷号 | 30期号:2页码:314-320 |
文章类型 | Article |
摘要 | Chitosan and carboxymethl-chitosan (CM-chitosan) membranes with different molecular mass were prepared by a casting method. The cytocompatibility of two kinds of polysaccharide membranes to skin fibroblasts that cultured in vitro were studied. The methods were to culture the cells in soaking fluid of membranes and to culture the cells on the membranes directly. The results showed that the soaking fluid had no toxicity to fibroblasts and the biological security of lower molecular mass membranes were better than higher molecular mass membranes, and CM-chitosan membranes were better than chitosan membranes. In addition, the growth of fibroblasts on chitosan membranes was inhibited and the cells would fall off from chitosan membranes after a period of culture. However, the cells adhered and expanded well on CM-chitosan membranes. All these demonstrated that cytocompatibility of CM-chitosan membranes to skin fibroblasts was better than chitosan membranes.; Chitosan and carboxymethl-chitosan (CM-chitosan) membranes with different molecular mass were prepared by a casting method. The cytocompatibility of two kinds of polysaccharide membranes to skin fibroblasts that cultured in vitro were studied. The methods were to culture the cells in soaking fluid of membranes and to culture the cells on the membranes directly. The results showed that the soaking fluid had no toxicity to fibroblasts and the biological security of lower molecular mass membranes were better than higher molecular mass membranes, and CM-chitosan membranes were better than chitosan membranes. In addition, the growth of fibroblasts on chitosan membranes was inhibited and the cells would fall off from chitosan membranes after a period of culture. However, the cells adhered and expanded well on CM-chitosan membranes. All these demonstrated that cytocompatibility of CM-chitosan membranes to skin fibroblasts was better than chitosan membranes. |
关键词 | Chitosan Carboxymethl-chitosan (Cm-chitosan) Membrane Skin Fibroblast Cytocompatibility |
学科领域 | Biochemistry & Molecular Biology ; Biophysics |
收录类别 | SCI |
语种 | 英语 |
WOS记录号 | WOS:000182764700028 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.qdio.ac.cn/handle/337002/2808 |
专题 | 海洋生态与环境科学重点实验室 |
作者单位 | 1.Ocean Univ Qingdao, Life Sci Coll, Qingdao 266003, Peoples R China 2.Chinese Acad Sci, Grad Sch, Inst Oceanol, Qingdao 266071, Peoples R China |
推荐引用方式 GB/T 7714 | Zheng, L,Chen, XG,Liu, WS,et al. Study on cytocompatibility of carboxymethyl-chitosan membranes to skin fibroblasts[J]. PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,2003,30(2):314-320. |
APA | Zheng, L,Chen, XG,Liu, WS,Han, XT,&Yan, XJ.(2003).Study on cytocompatibility of carboxymethyl-chitosan membranes to skin fibroblasts.PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,30(2),314-320. |
MLA | Zheng, L,et al."Study on cytocompatibility of carboxymethyl-chitosan membranes to skin fibroblasts".PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS 30.2(2003):314-320. |
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