Osmoregulatory actions of growth hormone in juvenile tilapia (Oreochromis niloticus)

	Osmoregulatory actions of growth hormone in juvenile tilapia (Oreochromis niloticus)
	Xu, B ; Miao, H ; Zhang, P; Li, D
	1997-12-01
发表期刊	FISH PHYSIOLOGY AND BIOCHEMISTRY
ISSN	0920-1742
卷号	17 期号:1-6 页码:295-301
文章类型	Article
摘要	Growth hormone (GH) effectively promotes seawater (SW) adaptation in salmonids, but little is known of its effect in tilapias. Experiments were performed to investigate the effects of recombinant eel GH (reGH) on osmoregulatory actions and ultrastructural features of gill chloride cells in juvenile tilapia, Oreochromis niloticus. Tilapia showed a markedly improved SW survival, when directly transferred from freshwater (FW) to 62.5% SW 24h after a single reGH injection (0.25 or 2.5 mu g g(-1)) or 3 reGH injections (0.25 mu g g(-1) every other day). Plasma Na+ and Mg2+ levels were significantly reduced by reGH (0.25 and 2.5 mu g g(-1)) compared with saline injections; Ca2+ concentrations were reduced significantly by high dose of reGH (2.5 mu g g(-1)) after SW transfer. However, fish failed to survive more than 24h when directly transferred to 70 % SW, although the fish treated with reGH could survive longer than the controls. When examined by electron microscopy, the chloride cells were identified as mitochondrion-rich and an extensive tubular system was induced by GH treatment. The results of the present study suggest that, similar to its effect on salmonids, GH also exerts acute osmoregulatory actions and enhances SW adaptation in juvenile tilapia. GH also stimulates the differentiation of chloride cells toward SW adaptation.; Growth hormone (GH) effectively promotes seawater (SW) adaptation in salmonids, but little is known of its effect in tilapias. Experiments were performed to investigate the effects of recombinant eel GH (reGH) on osmoregulatory actions and ultrastructural features of gill chloride cells in juvenile tilapia, Oreochromis niloticus. Tilapia showed a markedly improved SW survival, when directly transferred from freshwater (FW) to 62.5% SW 24h after a single reGH injection (0.25 or 2.5 mu g g(-1)) or 3 reGH injections (0.25 mu g g(-1) every other day). Plasma Na+ and Mg2+ levels were significantly reduced by reGH (0.25 and 2.5 mu g g(-1)) compared with saline injections; Ca2+ concentrations were reduced significantly by high dose of reGH (2.5 mu g g(-1)) after SW transfer. However, fish failed to survive more than 24h when directly transferred to 70 % SW, although the fish treated with reGH could survive longer than the controls. When examined by electron microscopy, the chloride cells were identified as mitochondrion-rich and an extensive tubular system was induced by GH treatment. The results of the present study suggest that, similar to its effect on salmonids, GH also exerts acute osmoregulatory actions and enhances SW adaptation in juvenile tilapia. GH also stimulates the differentiation of chloride cells toward SW adaptation.
关键词	Tilapia Oreochromis Niloticus Osmoregulation Growth Hormone Seawater Adaptation Plasma Ions Gill Chloride Cells Mitochondrion-rich Tubular System
学科领域	Biochemistry & Molecular Biology ; Fisheries ; Physiology
收录类别	ISTP ; SCI
语种	英语
WOS记录号	WOS:000071981100037
引用统计	被引频次：23[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/2868
专题	海洋生态与环境科学重点实验室实验海洋生物学重点实验室
作者单位	1.Ocean Univ Qingdao, Coll Fisheries, Qingdao 266003, Peoples R China 2.Ocean Univ Qingdao, Marine Life Sci Coll, Qingdao 266003, Peoples R China 3.Acad Sinica, Inst Oceanol, Lab Expt Marine Biol, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Xu, B,Miao, H,Zhang, P,et al. Osmoregulatory actions of growth hormone in juvenile tilapia (Oreochromis niloticus)[J]. FISH PHYSIOLOGY AND BIOCHEMISTRY,1997,17(1-6):295-301.
APA	Xu, B,Miao, H,Zhang, P,&Li, D.(1997).Osmoregulatory actions of growth hormone in juvenile tilapia (Oreochromis niloticus).FISH PHYSIOLOGY AND BIOCHEMISTRY,17(1-6),295-301.
MLA	Xu, B,et al."Osmoregulatory actions of growth hormone in juvenile tilapia (Oreochromis niloticus)".FISH PHYSIOLOGY AND BIOCHEMISTRY 17.1-6(1997):295-301.

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