Functional biosynthesis of an allophycocyan beta subunit in Escherichia coli

doi:10.1016/j.jbiosc.2008.11.014

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	Functional biosynthesis of an allophycocyan beta subunit in Escherichia coli
	Ge, Baosheng 1,2; Sun, Haixiang 3; Feng, Yang 1; Yang, Jinying 1; Qin, Song 4
	2009-03-01
发表期刊	JOURNAL OF BIOSCIENCE AND BIOENGINEERING
ISSN	1389-1723
卷号	107 期号:3 页码:246-249
文章类型	Article
摘要	Allophycocyanin is a phycobiliprotein with various biological and pharmacological properties. An expression vector was constructed using CpeS as the bilin lyase for the allophycocyanin beta subunit, resulting in overexpression of a fluorescent allophycocyanin beta-subunit in Escherichia coli. A high-density cell culture was developed using a continuous feeding strategy. After 16 h of culture, the dry cell density reached 21.4 g 1(-1), the expression of the allophycocyanin beta-subunit was 0.86 g l(-1) broth, and the relative chromoprotein yield was 81.4%. The recombinant protein showed spectral features similar to native allophycocyanin, which provide an efficient methodology for large-scale production of this valuable fluorescent protein. (C) 2008, The Society for Biotechnology, Japan. All rights reserved.; Allophycocyanin is a phycobiliprotein with various biological and pharmacological properties. An expression vector was constructed using CpeS as the bilin lyase for the allophycocyanin beta subunit, resulting in overexpression of a fluorescent allophycocyanin beta-subunit in Escherichia coli. A high-density cell culture was developed using a continuous feeding strategy. After 16 h of culture, the dry cell density reached 21.4 g 1(-1), the expression of the allophycocyanin beta-subunit was 0.86 g l(-1) broth, and the relative chromoprotein yield was 81.4%. The recombinant protein showed spectral features similar to native allophycocyanin, which provide an efficient methodology for large-scale production of this valuable fluorescent protein. (C) 2008, The Society for Biotechnology, Japan. All rights reserved.
关键词	Allophycocyanin Beta Subunit Bilin Lyase Biosynthesis Chromophore Escherichia Coli
学科领域	Biotechnology & Applied Microbiology ; Food Science & Technology
DOI	10.1016/j.jbiosc.2008.11.014
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000265311200006
引用统计	被引频次：15[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/2912
专题	实验海洋生物学重点实验室
作者单位	1.China Univ Petr, Ctr Biotechnol & Bioengn, Qingdao 266555, Shandong, Peoples R China 2.Huaihai Inst Technol, Jiangsu Key Lab Marine Biotechnol, Lianyungang 222005, Jiangsu, Peoples R China 3.China Univ Petr, Coll Chem & Chem Engn, Dongying 257061, Shandong, Peoples R China 4.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Shandong, Peoples R China
推荐引用方式 GB/T 7714	Ge, Baosheng,Sun, Haixiang,Feng, Yang,et al. Functional biosynthesis of an allophycocyan beta subunit in Escherichia coli[J]. JOURNAL OF BIOSCIENCE AND BIOENGINEERING,2009,107(3):246-249.
APA	Ge, Baosheng,Sun, Haixiang,Feng, Yang,Yang, Jinying,&Qin, Song.(2009).Functional biosynthesis of an allophycocyan beta subunit in Escherichia coli.JOURNAL OF BIOSCIENCE AND BIOENGINEERING,107(3),246-249.
MLA	Ge, Baosheng,et al."Functional biosynthesis of an allophycocyan beta subunit in Escherichia coli".JOURNAL OF BIOSCIENCE AND BIOENGINEERING 107.3(2009):246-249.

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