Ontogenetic development of digestive enzymes and effect of starvation in miiuy croaker Miichthys miiuy larvae

doi:10.1007/s10695-008-9263-9

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	Ontogenetic development of digestive enzymes and effect of starvation in miiuy croaker Miichthys miiuy larvae
	Shan, Xiu-Juan 1,2; Huang, Wei 1,2; Cao, Liang 1,2; Xiao, Zhi-Zhong 1; Dou, Shuo-Zeng 1
	2009-08-01
发表期刊	FISH PHYSIOLOGY AND BIOCHEMISTRY
ISSN	0920-1742
卷号	35 期号:3 页码:385-398
文章类型	Article
摘要	The ontogenetic development of the digestive enzymes amylase, lipase, trypsin, and alkaline phosphatase and the effect of starvation in miiuy croaker Miichthys miiuy larvae were studied. The activities of these enzymes were detected prior to exogenous feeding, but their developmental patterns differed remarkably. Trypsin activity continuously increased from 2 days after hatching (dah), peaked on 20 dah, and decreased to 25 dah at weaning. Alkaline phosphatase activity oscillated at low levels within a small range after the first feeding on 3 dah. In contrast, amylase and lipase activities followed the general developmental pattern that has been characterized in fish larvae, with a succession of increases or decreases. Amylase, lipase, and trypsin activities generally started to increase or decrease at transitions from endogenous to exogenous feeding or diet changes, suggesting that these enzymatic activities can be modulated by feeding modes. The activities of all the enzymes remained stable from 25 dah onwards, coinciding with the formation of gastric glands and pyloric caecum. These results imply that specific activities of these enzymes underwent changes due to morphological and physiological modifications or diet shift during larval development but that they became stable after the development of the digestive organs and associated glands was fully completed and the organs/glands functioned. Trypsin and alkaline phosphatase were more sensitive to starvation than amylase and lipase because delayed feeding up to 2 days after mouth opening was able to adversely affect their activities. Enzyme activities did not significantly differ among feeding groups during endogenous feeding; however, all activities were remarkably reduced when delayed feeding was within 3 days after mouth opening. Initiation of larvae feeding should occur within 2 days after mouth opening so that good growth and survival can be obtained in the culture.; The ontogenetic development of the digestive enzymes amylase, lipase, trypsin, and alkaline phosphatase and the effect of starvation in miiuy croaker Miichthys miiuy larvae were studied. The activities of these enzymes were detected prior to exogenous feeding, but their developmental patterns differed remarkably. Trypsin activity continuously increased from 2 days after hatching (dah), peaked on 20 dah, and decreased to 25 dah at weaning. Alkaline phosphatase activity oscillated at low levels within a small range after the first feeding on 3 dah. In contrast, amylase and lipase activities followed the general developmental pattern that has been characterized in fish larvae, with a succession of increases or decreases. Amylase, lipase, and trypsin activities generally started to increase or decrease at transitions from endogenous to exogenous feeding or diet changes, suggesting that these enzymatic activities can be modulated by feeding modes. The activities of all the enzymes remained stable from 25 dah onwards, coinciding with the formation of gastric glands and pyloric caecum. These results imply that specific activities of these enzymes underwent changes due to morphological and physiological modifications or diet shift during larval development but that they became stable after the development of the digestive organs and associated glands was fully completed and the organs/glands functioned. Trypsin and alkaline phosphatase were more sensitive to starvation than amylase and lipase because delayed feeding up to 2 days after mouth opening was able to adversely affect their activities. Enzyme activities did not significantly differ among feeding groups during endogenous feeding; however, all activities were remarkably reduced when delayed feeding was within 3 days after mouth opening. Initiation of larvae feeding should occur within 2 days after mouth opening so that good growth and survival can be obtained in the culture.
关键词	Amylase Alkaline Phosphatase Lipase Trypsin Delayed Feeding Miichthys Miiuy Larvae
学科领域	Biochemistry & Molecular Biology ; Fisheries ; Physiology
DOI	10.1007/s10695-008-9263-9
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000267681000009
引用统计	被引频次：25[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/3006
专题	实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Shan, Xiu-Juan,Huang, Wei,Cao, Liang,et al. Ontogenetic development of digestive enzymes and effect of starvation in miiuy croaker Miichthys miiuy larvae[J]. FISH PHYSIOLOGY AND BIOCHEMISTRY,2009,35(3):385-398.
APA	Shan, Xiu-Juan,Huang, Wei,Cao, Liang,Xiao, Zhi-Zhong,&Dou, Shuo-Zeng.(2009).Ontogenetic development of digestive enzymes and effect of starvation in miiuy croaker Miichthys miiuy larvae.FISH PHYSIOLOGY AND BIOCHEMISTRY,35(3),385-398.
MLA	Shan, Xiu-Juan,et al."Ontogenetic development of digestive enzymes and effect of starvation in miiuy croaker Miichthys miiuy larvae".FISH PHYSIOLOGY AND BIOCHEMISTRY 35.3(2009):385-398.

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