A cyclophilin A inducible expressed in gonad of zhikong scallop Chlamys farreri

doi:10.1007/s11033-008-9363-8

IOCAS-IR > 实验海洋生物学重点实验室

	A cyclophilin A inducible expressed in gonad of zhikong scallop Chlamys farreri
	Song, Xiaoyan 1,2; Wang, Lingling 1; Song, Linsheng 1; Zhao, Jianmin 1; Zhang, Huan 1; Zheng, Peilin 1; Qiu, Limei 1; Liu, Xiaolin 2; Wu, Longtao 1
	2009-07-01
发表期刊	MOLECULAR BIOLOGY REPORTS
ISSN	0301-4851
卷号	36 期号:6 页码:1637-1645
文章类型	Article
摘要	Cyclophilin A (CypA), a receptor for the immunosuppressive agent cyclosporin A (CsA), is a cis-trans peptidyl-prolyl isomerase (PPIase) which accelerates the cis-trans isomerization of prolyl-peptide bonds, interacts with a variety of proteins and therefore regulates their activities. One CypA (designated CfCypA) cDNA was cloned from Chlamys farreri by expressed sequence tag (EST) and rapid amplification of cDNA ends (RACE) techniques. The full-length cDNA of CfCypA consisted of 1,248 nucleotides with a canonical polyadenylation signal sequence AATAAA, a poly (A) tail, and an open reading frame (ORF) of 495 nucleotides encoding a polypeptide of 164 amino acids. The deduced amino acid sequence shared high similarity with CypA from the other species, indicating that CfCypA should be a new member of the CypA family. Quantitative real-time (RT) PCR was employed to assess the mRNA expression of CfCypA in various tissues and its temporal expression in haemocytes and gonad of scallops challenged with Vibrio anguillarum. The mRNA transcripts of CfCypA could be detected in all the examined tissues with highest expression level in gonad. After bacterial challenge, the expression level of CfCypA was almost unchanged in haemocytes, but up-regulated in gonad and increased to the peak (22.59-fold; P < 0.05) at 4 h post-injection, and then dropped to the original level at 8 h post-injection. These results indicated that CfCypA was constitutive expressed in haemocytes, but could be induced in gonad, and perhaps played a critical role in response to the bacterial challenge in gonad.; Cyclophilin A (CypA), a receptor for the immunosuppressive agent cyclosporin A (CsA), is a cis-trans peptidyl-prolyl isomerase (PPIase) which accelerates the cis-trans isomerization of prolyl-peptide bonds, interacts with a variety of proteins and therefore regulates their activities. One CypA (designated CfCypA) cDNA was cloned from Chlamys farreri by expressed sequence tag (EST) and rapid amplification of cDNA ends (RACE) techniques. The full-length cDNA of CfCypA consisted of 1,248 nucleotides with a canonical polyadenylation signal sequence AATAAA, a poly (A) tail, and an open reading frame (ORF) of 495 nucleotides encoding a polypeptide of 164 amino acids. The deduced amino acid sequence shared high similarity with CypA from the other species, indicating that CfCypA should be a new member of the CypA family. Quantitative real-time (RT) PCR was employed to assess the mRNA expression of CfCypA in various tissues and its temporal expression in haemocytes and gonad of scallops challenged with Vibrio anguillarum. The mRNA transcripts of CfCypA could be detected in all the examined tissues with highest expression level in gonad. After bacterial challenge, the expression level of CfCypA was almost unchanged in haemocytes, but up-regulated in gonad and increased to the peak (22.59-fold; P < 0.05) at 4 h post-injection, and then dropped to the original level at 8 h post-injection. These results indicated that CfCypA was constitutive expressed in haemocytes, but could be induced in gonad, and perhaps played a critical role in response to the bacterial challenge in gonad.
关键词	Scallop Chlamys Farreri Cyclophilin a Mrna Expression Quantitative Real-time Pcr Bacteria Challenge
学科领域	Biochemistry & Molecular Biology
DOI	10.1007/s11033-008-9363-8
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000266454500061
引用统计	被引频次：23[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/3010
专题	实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Shandong, Peoples R China 2.NW A&F Univ, Coll Anim Sci & Technol, Yangling 712100, Shaanxi, Peoples R China
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Song, Xiaoyan,Wang, Lingling,Song, Linsheng,et al. A cyclophilin A inducible expressed in gonad of zhikong scallop Chlamys farreri[J]. MOLECULAR BIOLOGY REPORTS,2009,36(6):1637-1645.
APA	Song, Xiaoyan.,Wang, Lingling.,Song, Linsheng.,Zhao, Jianmin.,Zhang, Huan.,...&Wu, Longtao.(2009).A cyclophilin A inducible expressed in gonad of zhikong scallop Chlamys farreri.MOLECULAR BIOLOGY REPORTS,36(6),1637-1645.
MLA	Song, Xiaoyan,et al."A cyclophilin A inducible expressed in gonad of zhikong scallop Chlamys farreri".MOLECULAR BIOLOGY REPORTS 36.6(2009):1637-1645.

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
song-A cyclophilin A（788KB）			限制开放	--	浏览