Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
Identification of an Atlantic salmon IFN multigene cluster encoding three IFN subtypes with very different expression properties | |
Sun, Baojian1; Robertsen, Borre1; Wang, Zhiqiang2,3; Liu, Bin2,3 | |
2009 | |
发表期刊 | DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY |
ISSN | 0145-305X |
卷号 | 33期号:4页码:547-558 |
文章类型 | Article |
摘要 | A cluster of 11 interferon (IFN) genes were identified in the Atlantic salmon genome linked to the growth hormone I gene. The genes encode three different IFN subtypes; IFNa (two genes), IFNb (four genes) and IFNc (five genes), which show 22-32% amino acid sequence identity. Expression of the fish IFNs were studied in head kidney, leukocytes or To cells after stimulation with the dsRNA poly I:C or the imidazoquinoline S-27609. In mammals, poly I:C induces IFN-beta through the RIG-I/MDA5 or the TLR3 pathway, both of which are dependent on NF-kappa B. In contrast, S-27609 induces mammalian IFN-alpha in plasmacytoid dendritic cells through the TLR7 pathway independent of NF-kappa B. The presence of an NF-kappa B site in their promoters and their strong up-regulation by poly I:C, suggest that salmon IFNa1/IFNa2 are induced through similar pathways as IFN-beta. In contrast, the apparent lack of NF-kappa B motif in the promoter and the strong upregulation by S-27609 in head kidney and leukocytes, suggest that IFNb genes are induced through a pathway similar to mammalian IFN-alpha. IFNc genes showed expression patterns different from both IFNa and IFNb. Taken together, salmon IFNa and IFNb are not orthologs of mammalian IFN-beta and IFN-alpha, respectively, but appear to utilize similar induction pathways. (C) 2008 Elsevier Ltd. All rights reserved.; A cluster of 11 interferon (IFN) genes were identified in the Atlantic salmon genome linked to the growth hormone I gene. The genes encode three different IFN subtypes; IFNa (two genes), IFNb (four genes) and IFNc (five genes), which show 22-32% amino acid sequence identity. Expression of the fish IFNs were studied in head kidney, leukocytes or To cells after stimulation with the dsRNA poly I:C or the imidazoquinoline S-27609. In mammals, poly I:C induces IFN-beta through the RIG-I/MDA5 or the TLR3 pathway, both of which are dependent on NF-kappa B. In contrast, S-27609 induces mammalian IFN-alpha in plasmacytoid dendritic cells through the TLR7 pathway independent of NF-kappa B. The presence of an NF-kappa B site in their promoters and their strong up-regulation by poly I:C, suggest that salmon IFNa1/IFNa2 are induced through similar pathways as IFN-beta. In contrast, the apparent lack of NF-kappa B motif in the promoter and the strong upregulation by S-27609 in head kidney and leukocytes, suggest that IFNb genes are induced through a pathway similar to mammalian IFN-alpha. IFNc genes showed expression patterns different from both IFNa and IFNb. Taken together, salmon IFNa and IFNb are not orthologs of mammalian IFN-beta and IFN-alpha, respectively, but appear to utilize similar induction pathways. (C) 2008 Elsevier Ltd. All rights reserved. |
关键词 | Interferon Promoter Antiviral Fish Reptile Anolis Dsrna Virus Evolution |
学科领域 | Immunology ; Zoology |
DOI | 10.1016/j.dci.2008.10.001 |
URL | 查看原文 |
收录类别 | SCI |
语种 | 英语 |
WOS记录号 | WOS:000263781100016 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.qdio.ac.cn/handle/337002/3016 |
专题 | 实验海洋生物学重点实验室 |
作者单位 | 1.Univ Tromso, Norwegian Coll Fishery Sci, Dept Marine Biotechnol, N-9037 Tromso, Norway 2.Chinese Acad Sci, Inst Oceanol, Qingdao, Peoples R China 3.Chinese Acad Sci, Beijing Inst Genom, Beijing, Peoples R China |
推荐引用方式 GB/T 7714 | Sun, Baojian,Robertsen, Borre,Wang, Zhiqiang,et al. Identification of an Atlantic salmon IFN multigene cluster encoding three IFN subtypes with very different expression properties[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2009,33(4):547-558. |
APA | Sun, Baojian,Robertsen, Borre,Wang, Zhiqiang,&Liu, Bin.(2009).Identification of an Atlantic salmon IFN multigene cluster encoding three IFN subtypes with very different expression properties.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,33(4),547-558. |
MLA | Sun, Baojian,et al."Identification of an Atlantic salmon IFN multigene cluster encoding three IFN subtypes with very different expression properties".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 33.4(2009):547-558. |
条目包含的文件 | ||||||
文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
sun-Identification o(2418KB) | 限制开放 | -- | 浏览 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。
修改评论