Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| Purification and characterization of the chitosanase from Aeromonas sp HG08 | |
| Sun, Yuying2; Zhang, Jiquan1; Wang, Shujun2 | |
| 2009-06-17 | |
| 发表期刊 | AFRICAN JOURNAL OF BIOTECHNOLOGY
 |
| ISSN | 1684-5315 |
| 卷号 | 8期号:12页码:2830-2834 |
| 文章类型 | Article |
| 摘要 | In this work, the characterization of a chitosanase-producing bacterium isolated from soil was reported and this strain was grouped under the genus Aeromonas by virtue of its morphological, physiological properties and 16S rDNA gene sequences. It is the first report that the genus Aeromonas could produce chitosanase. Aeromonas sp. HG08 could secrete the chitosanase ( named AsChi) with molecular weight of 70 kDa. The optimum pH and temperature of AsChi was 6.0 and 55 degrees C, respectively. The activity of AsChi was markedly enhanced by Mn(2+) and inhibited by Fe(3+), Cu(2+), Ag(+) and Hg(2+); additionally, the activity of AsChi was increased with the degree of deacetylation ( DDA) of chitosan. Through viscosimetric assay, AsChi probably hydrolyzed chitosan in an endo-type fashion.; In this work, the characterization of a chitosanase-producing bacterium isolated from soil was reported and this strain was grouped under the genus Aeromonas by virtue of its morphological, physiological properties and 16S rDNA gene sequences. It is the first report that the genus Aeromonas could produce chitosanase. Aeromonas sp. HG08 could secrete the chitosanase ( named AsChi) with molecular weight of 70 kDa. The optimum pH and temperature of AsChi was 6.0 and 55 degrees C, respectively. The activity of AsChi was markedly enhanced by Mn2+ and inhibited by Fe3+, Cu2+, Ag+ and Hg2+; additionally, the activity of AsChi was increased with the degree of deacetylation ( DDA) of chitosan. Through viscosimetric assay, AsChi probably hydrolyzed chitosan in an endo-type fashion. |
| 关键词 | Aeromonas Sp. Chitosanase Purification Characterization |
| 学科领域 | Biotechnology & Applied Microbiology |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000268831200028 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/3020 |
| 专题 | 实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Huaihai Inst Technol, Coll Marine Sci, Lianyungang 222005, Peoples R China |
| 推荐引用方式 GB/T 7714 | Sun, Yuying,Zhang, Jiquan,Wang, Shujun. Purification and characterization of the chitosanase from Aeromonas sp HG08[J]. AFRICAN JOURNAL OF BIOTECHNOLOGY,2009,8(12):2830-2834. |
| APA | Sun, Yuying,Zhang, Jiquan,&Wang, Shujun.(2009).Purification and characterization of the chitosanase from Aeromonas sp HG08.AFRICAN JOURNAL OF BIOTECHNOLOGY,8(12),2830-2834. |
| MLA | Sun, Yuying,et al."Purification and characterization of the chitosanase from Aeromonas sp HG08".AFRICAN JOURNAL OF BIOTECHNOLOGY 8.12(2009):2830-2834. |
| 条目包含的文件 | ||||||
| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| sun-Purification and（104KB） | 限制开放 | -- | 浏览 | |||
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