Identification of a C-type lectin from the bay scallop Argopecten irradians

doi:10.1007/s11033-008-9293-5

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	Identification of a C-type lectin from the bay scallop Argopecten irradians
	Zhu, Ling 1,2,3; Song, Linsheng 4; Xu, Wei 4; Qian, Pei-Yuan 1,2,3
	2009-05-01
发表期刊	MOLECULAR BIOLOGY REPORTS
ISSN	0301-4851
卷号	36 期号:5 页码:1167-1173
文章类型	Article
摘要	C-type lectins are Ca(2+) dependent carbohydrate-recognition proteins that play crucial roles in the invertebrate innate immunity, such as nonself recognition, activation of proPO system, antibacterial activity, promotion of phagocytosis and nodule formation. In this study, a novel C-type lectin of bay scallops Argopecten irradians (Ai Lec) was identified using expressed sequence tag (EST) and RACE techniques. The Ai Lec cDNA encoded a polypeptide of 171 amino acids with a putative signal peptide of 21 amino acid residues and a mature protein of 150 amino acids. The deduced amino acid sequence of Ai Lec was highly similar to those of the C-type lectins from other animals and contained a typical carbohydrate-recognition domain (CRD) of 131 residues, which has four conserved disulfide-bonded cysteine residues that define the CRD and two additional cysteine residues at the amino terminus. The expression of Ai Lec transcript was dominantly detected in the hepatopancreas and slightly detected in the haemocytes of normal scallops. 6 h after Vibrio anguillarum-challenge and 8 h after Micrococcus luteus-challenge, the temporal expression of Ai Lec mRNA in hemocytes was increased by 4.4- and 3.6-folds, respectively. The results suggested that Ai Lec was a constitutive and inducible acute-phase protein and might be involved in immune response to Gram-negative and Gram-positive microbial infection in bay scallop A. irradians.; C-type lectins are Ca2+ dependent carbohydrate-recognition proteins that play crucial roles in the invertebrate innate immunity, such as nonself recognition, activation of proPO system, antibacterial activity, promotion of phagocytosis and nodule formation. In this study, a novel C-type lectin of bay scallops Argopecten irradians (Ai Lec) was identified using expressed sequence tag (EST) and RACE techniques. The Ai Lec cDNA encoded a polypeptide of 171 amino acids with a putative signal peptide of 21 amino acid residues and a mature protein of 150 amino acids. The deduced amino acid sequence of Ai Lec was highly similar to those of the C-type lectins from other animals and contained a typical carbohydrate-recognition domain (CRD) of 131 residues, which has four conserved disulfide-bonded cysteine residues that define the CRD and two additional cysteine residues at the amino terminus. The expression of Ai Lec transcript was dominantly detected in the hepatopancreas and slightly detected in the haemocytes of normal scallops. 6 h after Vibrio anguillarum-challenge and 8 h after Micrococcus luteus-challenge, the temporal expression of Ai Lec mRNA in hemocytes was increased by 4.4- and 3.6-folds, respectively. The results suggested that Ai Lec was a constitutive and inducible acute-phase protein and might be involved in immune response to Gram-negative and Gram-positive microbial infection in bay scallop A. irradians.
关键词	C-type Lectin Crd Argopecten Irradians Tissue Distribution Mrna Expression Immune Response
学科领域	Biochemistry & Molecular Biology
DOI	10.1007/s11033-008-9293-5
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000264851600042
引用统计	被引频次：23[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/3082
专题	实验海洋生物学重点实验室
作者单位	1.Hong Kong Univ Sci & Technol, Dept Biol, Kowloon, Hong Kong, Peoples R China 2.Hong Kong Univ Sci & Technol, Coastal Marine Lab, Kowloon, Hong Kong, Peoples R China 3.Chinese Acad Sci, S China Sea Inst Oceanog, Key Lab Trop Marine Environm Dynam, Guangzhou 510301, Guangdong, Peoples R China 4.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Zhu, Ling,Song, Linsheng,Xu, Wei,et al. Identification of a C-type lectin from the bay scallop Argopecten irradians[J]. MOLECULAR BIOLOGY REPORTS,2009,36(5):1167-1173.
APA	Zhu, Ling,Song, Linsheng,Xu, Wei,&Qian, Pei-Yuan.(2009).Identification of a C-type lectin from the bay scallop Argopecten irradians.MOLECULAR BIOLOGY REPORTS,36(5),1167-1173.
MLA	Zhu, Ling,et al."Identification of a C-type lectin from the bay scallop Argopecten irradians".MOLECULAR BIOLOGY REPORTS 36.5(2009):1167-1173.

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