Isolation of the main light-harvesting chlorophyll a/b-protein complex from thylakoid membranes of marine alga, Bryopsis corticulans by a direct method

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	Isolation of the main light-harvesting chlorophyll a/b-protein complex from thylakoid membranes of marine alga, Bryopsis corticulans by a direct method
	Chen, H; Shen, SH; Wang, GC; Li, LB; Kuang, TY
	2004
发表期刊	ACTA BOTANICA SINICA
ISSN	1672-6650
卷号	46 期号:8 页码:915-920
摘要	The main chlorophyll a/b light-harvesting complex (LHC 11) has been isolated directly from thylakoid membranes of marine green alga (Bryopsis corticulans Setch.) by two consecutive runs of anion exchange and gel-filtration chromatography. LHC 11 proteins in the membrane extracts treated with 3% n-Octyl-b-D-glucopyranoside (OG) obtained specific binding ability on Q Sepharose column, and thus were isolated from the thylakoid membranes in a highly selective fraction. The monomeric, trimeric and oligomeric subcomplexes of LHC 11 have been obtained by fractionation of the LHC 11 mixes with sucrose density gradient ultracentrifugation. The SDS-PAGE analysis of peptide composition and absorption spectrum showed that LHC 11 monomers, trimers and oligomers prepared through this work were intact and in high purity. Our report is the first to show that it is possible to purify LHC If directly from thylakoid membranes without extensively biochemical purification.
关键词	Photosystem-ii Electron Crystallography Binding Proteins
学科领域	Biochemistry & Molecular Biology ; Plant Sciences
收录类别	sci
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/3182
专题	实验海洋生物学重点实验室
推荐引用方式 GB/T 7714	Chen, H,Shen, SH,Wang, GC,et al. Isolation of the main light-harvesting chlorophyll a/b-protein complex from thylakoid membranes of marine alga, Bryopsis corticulans by a direct method[J]. ACTA BOTANICA SINICA,2004,46(8):915-920.
APA	Chen, H,Shen, SH,Wang, GC,Li, LB,&Kuang, TY.(2004).Isolation of the main light-harvesting chlorophyll a/b-protein complex from thylakoid membranes of marine alga, Bryopsis corticulans by a direct method.ACTA BOTANICA SINICA,46(8),915-920.
MLA	Chen, H,et al."Isolation of the main light-harvesting chlorophyll a/b-protein complex from thylakoid membranes of marine alga, Bryopsis corticulans by a direct method".ACTA BOTANICA SINICA 46.8(2004):915-920.

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