Isolation and characterization of photosystem II of Porphyra yezoensis ueda

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	Isolation and characterization of photosystem II of Porphyra yezoensis ueda
	Gao, ZQ ; Wang, GC ; Tseng, CK
	2004-11-01
发表期刊	ACTA BIOCHIMICA ET BIOPHYSICA SINICA
ISSN	1672-9145
卷号	36 期号:11 页码:780-785
文章类型	Article
摘要	The thylakoid membranes were isolated and purified from gametophyte of Porphyrayezoensis Ueda (P yezoensis) by sucrose density gradient ultracentrifugation. After R yezoensis gametophyte thylakoid membranes were solubilized with SDS, the photosystem 11 (PSII) particles were isolated and purified. The activity of PSII particles was determined with DCIP (2,6-dichloroindophenol) photoreduction reaction. The composition of purified PSII particles was detected by SDS-PAGE. As a result, seven proteins including 55 kD protein, 47 kD protein, 43 kD protein, 33 kD protein, 31 kD protein, 29 kD protein, and 18 kD protein were found. Compared with PSII particles of higher plants and other algae, they were identified as D1/D2 complex, CP47, CP43, 33 kD protein, D1, D2 and cyt c-550 respectively. Besides, other three new proteins of 20 kD, 16 kD and 14 kD respectively were found. Among these extrinsic proteins, the 16 kD and 14 kD proteins had not been reported previously, and the 20 kD protein was found for the first time in multicellular red algae.; The thylakoid membranes were isolated and purified from gametophyte of Porphyrayezoensis Ueda (P yezoensis) by sucrose density gradient ultracentrifugation. After R yezoensis gametophyte thylakoid membranes were solubilized with SDS, the photosystem 11 (PSII) particles were isolated and purified. The activity of PSII particles was determined with DCIP (2,6-dichloroindophenol) photoreduction reaction. The composition of purified PSII particles was detected by SDS-PAGE. As a result, seven proteins including 55 kD protein, 47 kD protein, 43 kD protein, 33 kD protein, 31 kD protein, 29 kD protein, and 18 kD protein were found. Compared with PSII particles of higher plants and other algae, they were identified as D1/D2 complex, CP47, CP43, 33 kD protein, D1, D2 and cyt c-550 respectively. Besides, other three new proteins of 20 kD, 16 kD and 14 kD respectively were found. Among these extrinsic proteins, the 16 kD and 14 kD proteins had not been reported previously, and the 20 kD protein was found for the first time in multicellular red algae.
关键词	Red Algae Porphyra Yezoensis Ueda Photosystem Ii Polypeptide Composition Extrinsic Protein
学科领域	Biochemistry & Molecular Biology ; Biophysics
收录类别	SCI
语种	英语
WOS记录号	WOS:000225325300010
引用统计	被引频次：9[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/3194
专题	实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Gao, ZQ,Wang, GC,Tseng, CK. Isolation and characterization of photosystem II of Porphyra yezoensis ueda[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2004,36(11):780-785.
APA	Gao, ZQ,Wang, GC,&Tseng, CK.(2004).Isolation and characterization of photosystem II of Porphyra yezoensis ueda.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,36(11),780-785.
MLA	Gao, ZQ,et al."Isolation and characterization of photosystem II of Porphyra yezoensis ueda".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 36.11(2004):780-785.

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