Characterization of carotenoid hydroxylase gene promoter in Haematococcus pluvialis

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	Characterization of carotenoid hydroxylase gene promoter in Haematococcus pluvialis
	Meng, C. X.; Wei, W.; Su, Z-L ; Qin, S.
	2006-10-01
发表期刊	INDIAN JOURNAL OF BIOCHEMISTRY & BIOPHYSICS
卷号	43 期号:5 页码:284-288
文章类型	Article
摘要	Astaxanthin, a high-value ketocarotenoid is mainly used in fish aquaculture. It also has potential in human health due to its higher antioxidant capacity than beta-carotene and vitamin E. The unicellular green alga Haematococcus pluvialis is known to accumulate astaxanthin in response to environmental stresses, such as high light intensity and salt stress. Carotenoid hydroxylase plays a key role in astaxanthin biosynthesis in H. pleevialis. In this paper, we report the characterization of a promoter-like region (378 to -22 bp) of carotenoid hydroxylase gene by cloning, sequence analysis and functional verification of its 919 by 5'-flanking region in H. pluvialis. The 5'-flanking region was characterized using micro-particle bombardment method and transient expression of LacZ reporter gene. Results of sequence analysis showed that the 5'-flanking region might have putative cis-acting elements, such as ABA (abscisic acid)-responsive element (ABRE), C-repeat/dehydration responsive element (C-repeadDRE), ethylene-responsive element (ERE), heat-shock element (HSE), wound-responsive element (WUN-motif), gibberellin-responsive element (P-box), MYB-binding site (MBS) etc., except for typical TATA and CCAAT boxes. Results of 5' deletions construct and beta-galactosidase assays revealed that a highest promoter-like region might exist from -378 to -22 by and some negative regulatory elements might lie in the region from -919 to -378 bp. Results of site-directed mutagenesis of a putative C-repeat/DRE and an ABRE-like motif in the promoterlike region (-378 to -22 bp) indicated that the putative C-repeatJDRE and ABRE-like motif might be important for expression of carotenoid hydroxylase gene.
关键词	Haematococcus Pluvialis Astaxanthin Carotenoid Hydroxylase Cis-acting Element Promoter
收录类别	SCI
语种	英语
WOS记录号	WOS:000241565800003
引用统计	被引频次：14[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/32554
专题	实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Meng, C. X.,Wei, W.,Su, Z-L,et al. Characterization of carotenoid hydroxylase gene promoter in Haematococcus pluvialis[J]. INDIAN JOURNAL OF BIOCHEMISTRY & BIOPHYSICS,2006,43(5):284-288.
APA	Meng, C. X.,Wei, W.,Su, Z-L,&Qin, S..(2006).Characterization of carotenoid hydroxylase gene promoter in Haematococcus pluvialis.INDIAN JOURNAL OF BIOCHEMISTRY & BIOPHYSICS,43(5),284-288.
MLA	Meng, C. X.,et al."Characterization of carotenoid hydroxylase gene promoter in Haematococcus pluvialis".INDIAN JOURNAL OF BIOCHEMISTRY & BIOPHYSICS 43.5(2006):284-288.

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