Molecular cloning and expression analysis of interleukin-1 beta from Japanese sea perch (Lateolabrax japonicus)

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	Molecular cloning and expression analysis of interleukin-1 beta from Japanese sea perch (Lateolabrax japonicus)
	Qiu, LH ; Song, LS ; Wu, LT ; Cai, ZH ; Jiang, SG
	2006
发表期刊	ACTA OCEANOLOGICA SINICA
卷号	25 期号:1 页码:127-136
文章类型	Article
摘要	The technology of homology cloning and anchored PCR was used to clone the IL-1 beta gene from the Japanese sea perch (Lateolabrax japonicus). The full-length cDNA of sea perch IL-1 beta was 1 310 bp, including a 5' untranslated region (UTR) of 136 bp, a 3' UTR of 430 bp, and an ORF of 774 bp encoding a polypeptide of 258 amino acids with an estimated molecular mass of 29.31 kDa. The searches for nucleotides and protein sequence similarities with the BLAST analysis indicated that the deduced amino acid sequence of sea perch IL-1 beta was homological to the IL-1 beta in other fish species and even the mammalian. Conserved signature sequences of the IL-1 beta gene family were found in the sea perch IL-1 beta deduced amino acid sequence. Temporal expressions of the IL-1 beta gene in LPS or iridovirus challenged group and in control group were measured by the semi-quantitative RT-PCR. The mRNA transcripts of IL-1 beta could be detected in head-kidney, spleen, liver, gill and heart of the healthy individuals, and the expression level of IL-1 beta in head-kidney, spleen and gill was higher than that in liver and heart, but it was hard to be detected in the brain. After being stimulated by the LPS or iridovirus, the IL-1 beta expression in most of examined tissues was up-regulated, and also could be detected in the brain. These results indicated that the expression of sea perch IL-1 beta was constitutive and could be up-regulated by immune effector stimulation. Therefore the sea perch IL-1 beta could play a critical role in the host-pathogen interaction.
关键词	Japanese Sea Perch Interleukin-1 Beta Gene Cloning Cdna Mrna Expression
收录类别	SCI
语种	英语
WOS记录号	WOS:000235984300013
引用统计	被引频次：1[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/32558
专题	实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Fishery Sci, S China Sea Fisheries Res Inst, Guangzhou 510300, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Qiu, LH,Song, LS,Wu, LT,et al. Molecular cloning and expression analysis of interleukin-1 beta from Japanese sea perch (Lateolabrax japonicus)[J]. ACTA OCEANOLOGICA SINICA,2006,25(1):127-136.
APA	Qiu, LH,Song, LS,Wu, LT,Cai, ZH,&Jiang, SG.(2006).Molecular cloning and expression analysis of interleukin-1 beta from Japanese sea perch (Lateolabrax japonicus).ACTA OCEANOLOGICA SINICA,25(1),127-136.
MLA	Qiu, LH,et al."Molecular cloning and expression analysis of interleukin-1 beta from Japanese sea perch (Lateolabrax japonicus)".ACTA OCEANOLOGICA SINICA 25.1(2006):127-136.

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Molecular cloning an（201KB）	期刊论文	出版稿	限制开放	ODC PDDL	浏览