Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| Genomic organization, nucleotide sequence analysis of the core histone genes cluster in Chlamys farreri and molecular evolution assessment of the H2A and H2B | |
| Li, Chenghua; Song, Linsheng; Zhao, Jianmin; Zou, Huibin; Su, Jianguo; Zhang, Huan | |
| 2006-12-01 | |
| 发表期刊 | DNA SEQUENCE
 |
| 卷号 | 17期号:6页码:440-451 |
| 文章类型 | Article |
| 摘要 | This work represents the nucleotide sequence of the core histone gene cluster from scallop Chlamys farreri. The tandemly repeated unit of 5671 bp containing a copy of the four core histone genes H4, H2B, H2A and H3 was amplified and identified by the techniques of homology cloning and genomic DNA walking. All the histone genes in the cluster had the structures in their 3' flanking region which related to the evolution of histone gene expression patterns throughout the cell cycle, including two different termination signals, the hairpin structure and at least one AATAAA polyadenylation signal. In their 5' region, the transcription initiation sites with a conserved sequence of 5'-P(y)CATTCP(u)-3' known as the CAP site were present in all genes except to H2B, generally 37-45 bp upstream of the start code. Canonical TATA and CAAT boxes were identified only in certain histone genes. In the case of the promoters of H2B and H2A genes, there was a 5'-GATCC-3' element, which had been found to be essential to start transcription at the appropriate site. After this element, in the promoter of H2B, there was another sequence, 5-GGATCGAAACGTTC-3', which was similar to the consensus sequence of 5'-GGAATAAACGTATTC-3' corresponding to the H2B-specific promoter element. The presence of enhancer sequences (5'-TGATATATG-3') was identified from the H4 and H3 genes, matching perfectly with the consensus sequence defined for histone genes. There were several slightly more complex repetitive DNA in the intergene regions. The presence of the series of conserved sequences and reiterated sequences was consistent with the view that mollusc histone gene cluster arose by duplicating of an ancestral precursor histone gene, the birth-and-death evolution model with strong purifying selection enabled the histone cluster less variation and more conserved function. Meanwhile, the H2A and the H2B were demonstrated to be potential good marks for phylogenetic analysis. All the results will be contributed to the characterization of repeating histone gene families in molluscs. |
| 关键词 | Chlamys Farreri Gene Cloning Histone Gene Cluster Phylogenetic Analysis |
| DOI | 10.1080/10425170600752593 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000243495500006 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/32582 |
| 专题 | 实验海洋生物学重点实验室 |
| 作者单位 | 1.Acad Sinica, Inst Oceanol, Qingdao 266071, Peoples R China 2.Acad Sinica, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Chenghua,Song, Linsheng,Zhao, Jianmin,et al. Genomic organization, nucleotide sequence analysis of the core histone genes cluster in Chlamys farreri and molecular evolution assessment of the H2A and H2B[J]. DNA SEQUENCE,2006,17(6):440-451. |
| APA | Li, Chenghua,Song, Linsheng,Zhao, Jianmin,Zou, Huibin,Su, Jianguo,&Zhang, Huan.(2006).Genomic organization, nucleotide sequence analysis of the core histone genes cluster in Chlamys farreri and molecular evolution assessment of the H2A and H2B.DNA SEQUENCE,17(6),440-451. |
| MLA | Li, Chenghua,et al."Genomic organization, nucleotide sequence analysis of the core histone genes cluster in Chlamys farreri and molecular evolution assessment of the H2A and H2B".DNA SEQUENCE 17.6(2006):440-451. |
| 条目包含的文件 | ||||||
| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| Genomic organization（781KB） | 期刊论文 | 出版稿 | 限制开放 | ODC PDDL | 浏览 | |
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。
修改评论