Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| Purification and primary identification of haemocyanin in the Chinese shrimp, Fenneropenaeus chinensis (Decapoda, Penaeoidea) | |
Huang, Bingxin1,2; Zhang, Jiquan1; Xiang, Jianhai1
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| 2008 | |
| 发表期刊 | CRUSTACEANA
 |
| 卷号 | 81期号:7页码:769-780 |
| 文章类型 | Article |
| 摘要 | We here report the isolation of haemocyanin, the crustacean oxygen transporter, from the haemolymph of the Chinese shrimp, Fenneropenaeus chinensis. We employed a Superdex 75 column, and the purified protein was demonstrated as homogeneous by Native-PAGE. The molecular mass of the purified haemocyanin was estimated to be 450 kDa using SDS-PAGE. The subsequent 2-DE suggested that it would be composed of three different subunits of 75-76 kDa each. Primary structure determination was performed by LC-ESI-MS and the data obtained were compared with the NCBI database. The result showed that these three subunits are identical to a partial fragment of haemocyanin from Litopenaeus vannamei. This research could be relevant to obtain pure haemocyanin, and will be helpful for further study on the structure and function of this large molecule. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000258155900001 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/32643 |
| 专题 | 实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 第一作者单位 | 实验海洋生物学重点实验室 |
| 推荐引用方式 GB/T 7714 | Huang, Bingxin,Zhang, Jiquan,Xiang, Jianhai. Purification and primary identification of haemocyanin in the Chinese shrimp, Fenneropenaeus chinensis (Decapoda, Penaeoidea)[J]. CRUSTACEANA,2008,81(7):769-780. |
| APA | Huang, Bingxin,Zhang, Jiquan,&Xiang, Jianhai.(2008).Purification and primary identification of haemocyanin in the Chinese shrimp, Fenneropenaeus chinensis (Decapoda, Penaeoidea).CRUSTACEANA,81(7),769-780. |
| MLA | Huang, Bingxin,et al."Purification and primary identification of haemocyanin in the Chinese shrimp, Fenneropenaeus chinensis (Decapoda, Penaeoidea)".CRUSTACEANA 81.7(2008):769-780. |
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| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| Purification and pri（243KB） | 期刊论文 | 出版稿 | 限制开放 | ODC PDDL | 浏览 | |
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