Expression of muscle-related genes and two MyoD genes during amphioxus notochord development

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	Expression of muscle-related genes and two MyoD genes during amphioxus notochord development
	Urano, A ; Suzuki, MM ; Zhang, P; Satoh, N ; Satoh, G
	2003-09-01
发表期刊	EVOLUTION & DEVELOPMENT
ISSN	1520-541X
卷号	5 期号:5 页码:447-458
文章类型	Article
摘要	The notochord is one of the diagnostic features of the phylum Chordata. Despite the similarities in the early morphogenetic patterns of the notochords of various chordates, they are strikingly distinct from one another at the histological level. The amphioxus notochord is one example of an evolutionary novelty because it is made up of muscle cells. Our previous expressed sequence tag analysis, targeting messenger RNAs expressed in the adult amphioxus notochord, demonstrated that many muscle-related genes are expressed there. To characterize amphioxus notochord cells and to gain insights into the myogenic program in the notochord, we determined the spatial and temporal expression patterns of these muscle-related genes during amphioxus development. We found that BbNA1 (notochord actin), Amphi-Trop I (troponin I), Amphi-TPmyosin (tropomyosin), Amphi-MHC2 (myosin heavy chain), Amphi-nMRLC (notochord-specific myosin regulatory light chain), AmphinTitin/MLCK (notochord-specific titin/myosin light chain kinase), Amphi-MLP/CRP3 (muscle LIM protein), and Amphi-nCalponin (notochord-specific calponin) are expressed with characteristic patterns in notochord cells, including the central cells, dorsally located cells, and ventrally located cells, suggesting that each notochord cell has a unique molecular architecture that may reflect its function. In addition, we characterized two MyoD genes (Amphi-MyoD1 and Amphi-MyoD2) to gain insight into the genetic circuitry governing the formation of the notochord muscle. One of the MyoD genes (Amphi-MyoD2) is expressed in the central notochord cells, and the coexistence of Amphi-MyoD2 transcripts along with the Amphi-MLP/CRP3 transcripts implies the participation of Amphi-MyoD2 in the myogenic program in the notochord muscle.; The notochord is one of the diagnostic features of the phylum Chordata. Despite the similarities in the early morphogenetic patterns of the notochords of various chordates, they are strikingly distinct from one another at the histological level. The amphioxus notochord is one example of an evolutionary novelty because it is made up of muscle cells. Our previous expressed sequence tag analysis, targeting messenger RNAs expressed in the adult amphioxus notochord, demonstrated that many muscle-related genes are expressed there. To characterize amphioxus notochord cells and to gain insights into the myogenic program in the notochord, we determined the spatial and temporal expression patterns of these muscle-related genes during amphioxus development. We found that BbNA1 (notochord actin), Amphi-Trop I (troponin I), Amphi-TPmyosin (tropomyosin), Amphi-MHC2 (myosin heavy chain), Amphi-nMRLC (notochord-specific myosin regulatory light chain), AmphinTitin/MLCK (notochord-specific titin/myosin light chain kinase), Amphi-MLP/CRP3 (muscle LIM protein), and Amphi-nCalponin (notochord-specific calponin) are expressed with characteristic patterns in notochord cells, including the central cells, dorsally located cells, and ventrally located cells, suggesting that each notochord cell has a unique molecular architecture that may reflect its function. In addition, we characterized two MyoD genes (Amphi-MyoD1 and Amphi-MyoD2) to gain insight into the genetic circuitry governing the formation of the notochord muscle. One of the MyoD genes (Amphi-MyoD2) is expressed in the central notochord cells, and the coexistence of Amphi-MyoD2 transcripts along with the Amphi-MLP/CRP3 transcripts implies the participation of Amphi-MyoD2 in the myogenic program in the notochord muscle.
关键词	Branchiostoma-lanceolatum Fine Structure Rat-brain Calponin Protein Contraction Brachyury Myogenesis Conservation Vertebrates
学科领域	Evolutionary Biology ; Developmental Biology ; Genetics & Heredity
收录类别	SCI
语种	英语
WOS记录号	WOS:000185487200002
引用统计	被引频次：30[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/3264
专题	实验海洋生物学重点实验室
作者单位	1.Kyoto Univ, Grad Sch Sci, Dept Zool, Sakyo Ku, Kyoto 6068502, Japan 2.Chinese Acad Sci, Inst Oceanol, Shandong, Peoples R China
推荐引用方式 GB/T 7714	Urano, A,Suzuki, MM,Zhang, P,et al. Expression of muscle-related genes and two MyoD genes during amphioxus notochord development[J]. EVOLUTION & DEVELOPMENT,2003,5(5):447-458.
APA	Urano, A,Suzuki, MM,Zhang, P,Satoh, N,&Satoh, G.(2003).Expression of muscle-related genes and two MyoD genes during amphioxus notochord development.EVOLUTION & DEVELOPMENT,5(5),447-458.
MLA	Urano, A,et al."Expression of muscle-related genes and two MyoD genes during amphioxus notochord development".EVOLUTION & DEVELOPMENT 5.5(2003):447-458.

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