| "Tandem duplication-random loss" is not a real feature of oyster mitochondrial genomes | |
| Ren, Jianfeng1,3; Liu, Xiao1; Zhang, Guofan1; Liu, Bin1,2; Guo, Ximing4 | |
| 2009-02-19 | |
| 发表期刊 | BMC GENOMICS
 |
| 卷号 | 10 |
| 文章类型 | Article |
| 摘要 | Duplications and rearrangements of coding genes are major themes in the evolution of mitochondrial genomes, bearing important consequences in the function of mitochondria and the fitness of organisms. Yu et al. (BMC Genomics 2008, 9: 477) reported the complete mt genome sequence of the oyster Crassostrea hongkongensis (16,475 bp) and found that a DNA segment containing four tRNA genes (trnK(1), trnC, trnQ(1) and trnN), a duplicated (rrnS) and a split rRNA gene (rrnL5') was absent compared with that of two other Crassostrea species. It was suggested that the absence was a novel case of "tandem duplication-random loss" with evolutionary significance. We independently sequenced the complete mt genome of three C. hongkongensis individuals, all of which were 18,622 bp and contained the segment that was missing in Yu et al.'s sequence. Further, we designed primers, verified sequences and demonstrated that the sequence loss in Yu et al.'s study was an artifact caused by placing primers in a duplicated region. The duplication and split of ribosomal RNA genes are unique for Crassostrea oysters and not lost in C. hongkongensis. Our study highlights the need for caution when amplifying and sequencing through duplicated regions of the genome. |
| DOI | 10.1186/1471-2164-10-84 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000264124200002 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/32650 |
| 专题 | 海洋生物技术研发中心 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Beijing Inst Genom, Beijing 101300, Peoples R China 3.Chinese Acad Sci, Grad Univ, Beijing 100039, Peoples R China 4.Rutgers State Univ, Inst Marine & Coastal Sci, Haskin Shellfish Res Lab, Port Norris, NJ 08349 USA |
| 第一作者单位 | 中国科学院海洋研究所 |
| 推荐引用方式 GB/T 7714 | Ren, Jianfeng,Liu, Xiao,Zhang, Guofan,et al. "Tandem duplication-random loss" is not a real feature of oyster mitochondrial genomes[J]. BMC GENOMICS,2009,10. |
| APA | Ren, Jianfeng,Liu, Xiao,Zhang, Guofan,Liu, Bin,&Guo, Ximing.(2009)."Tandem duplication-random loss" is not a real feature of oyster mitochondrial genomes.BMC GENOMICS,10. |
| MLA | Ren, Jianfeng,et al.""Tandem duplication-random loss" is not a real feature of oyster mitochondrial genomes".BMC GENOMICS 10(2009). |
| 条目包含的文件 | ||||||
| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| _Tandem duplication-（430KB） | 期刊论文 | 出版稿 | 限制开放 | ODC PDDL | 浏览 | |
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