Development of expressed sequence tag-derived microsatellite markers for Saccharina (Laminaria) japonica

doi:10.1007/s10811-009-9426-9

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	Development of expressed sequence tag-derived microsatellite markers for Saccharina (Laminaria) japonica
	Liu, Fuli 1,2; Wang, Xiuliang 1; Yao, Jianting 1; Fu, Wandong 1,2; Duan, Delin 1
	2010-02-01
发表期刊	JOURNAL OF APPLIED PHYCOLOGY
卷号	22 期号:1 页码:109-111
文章类型	Article
摘要	Expressed sequence tag-derived microsatellite markers (EST-SSR) were generated and characterized in Laminaria japonica using data mining from updated public EST databases and polymorphism testing. Fifty-eight of 578 ESTs (10.0%) containing various repeat motifs were used to design polymerase chain reaction (PCR) amplification primers. A total of 12 pairs of primer were generated and used in the PCR amplification. Alleles per locus ranged from two to ten (average of 5.7). The observed heterozygosities and expected heterozygosities were from 0.045 to 0.543 and from 0.056 to 0.814, respectively. All loci were in Hardy-Weinberg equilibrium and no linkage disequilibrium was detected. These robust, informative, and potentially transferable polymorphic markers appear suitable for population, genetic, parentage, and mapping studies of L. japonica.
关键词	Phaeophyta Microsatellite Est Molecular Marker
DOI	10.1007/s10811-009-9426-9
收录类别	SCI
语种	英语
WOS记录号	WOS:000274435300013
引用统计	被引频次：27[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/32794
专题	实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100059, Peoples R China
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Liu, Fuli,Wang, Xiuliang,Yao, Jianting,et al. Development of expressed sequence tag-derived microsatellite markers for Saccharina (Laminaria) japonica[J]. JOURNAL OF APPLIED PHYCOLOGY,2010,22(1):109-111.
APA	Liu, Fuli,Wang, Xiuliang,Yao, Jianting,Fu, Wandong,&Duan, Delin.(2010).Development of expressed sequence tag-derived microsatellite markers for Saccharina (Laminaria) japonica.JOURNAL OF APPLIED PHYCOLOGY,22(1),109-111.
MLA	Liu, Fuli,et al."Development of expressed sequence tag-derived microsatellite markers for Saccharina (Laminaria) japonica".JOURNAL OF APPLIED PHYCOLOGY 22.1(2010):109-111.

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