An immune responsive multidomain galectin from bay scallop Argopectens irradians

doi:10.1016/j.fsi.2009.11.016

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	An immune responsive multidomain galectin from bay scallop Argopectens irradians
	Song, Xiaoyan 1,2; Zhang, Huan 1; Zhao, Jianmin 1; Wang, Lingling 1; Qiu, Limei 1; Mu, Changkao 1; Liu, Xiaolin 2; Qiu, Lihua 3; Song, Linsheng 1
	2010-02-01
发表期刊	FISH & SHELLFISH IMMUNOLOGY
卷号	28 期号:2 页码:326-332
文章类型	Article
摘要	Galectins are a family of P-galactoside-binding lectins which play crucial roles in innate immunity of vertebrates and invertebrates. In the present study, the cDNA of a galectin with multiple carbohydrate-recognition domains (CRDs) was cloned from bay scallop Argopectens irradians (designated AiGal1) by expressed sequence tag (EST) and rapid amplification of cDNA ends (RACE) techniques. The full-length cDNA of AiGal1 was of 2235 nucleotides, encoding a polypeptide of 549 amino acids. SMART program analysis revealed that AiGal1 contained four galectin CRDs, and all the CRDs contained the two consensus motifs essential for ligand-binding. Quantitative real-time PCR was employed to investigate the tissue distribution of AiGal1 mRNA and temporal expression in haemocytes of scallops challenged with Vibrio anguillarum, Micrococcus luteus and Pichia pastoris. The AiGal1 mRNA could be detected in all tested tissues with the highest expression level in hepatopancreas. After challenged by V. anguillarum and M. luteus, the expression level of AiGal1 mRNA was both up-regulated and reached the maximum level at 9 h (1.52 fold, P < 0.05) and 18 h (2.89 fold, P < 0.01) post challenge, respectively. However, there was no significant difference in the mRNA expression of AiGal1 in haemocytes after P. pastoris challenge (P > 0.05). These results collectively indicated that AiGal1 was a new member of the galectin family and involved in the immune responses against bacterial infection. (C) 2009 Elsevier Ltd. All rights reserved.
关键词	Argopectens Irradians Galectin Mrna Expression Quantitative Real-time Pcr Bacteria And Yeast Challenge
DOI	10.1016/j.fsi.2009.11.016
收录类别	SCI
语种	英语
WOS记录号	WOS:000274721300011
引用统计	被引频次：50[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/32813
专题	实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.NW A&F Univ, Coll Anim Sci & Technol, Yangling 712100, Peoples R China 3.Chinese Acad Fishery Sci, S China Sea Fisheries Res Inst, Guangzhou 510300, Guangdong, Peoples R China
第一作者单位	实验海洋生物学重点实验室
推荐引用方式 GB/T 7714	Song, Xiaoyan,Zhang, Huan,Zhao, Jianmin,et al. An immune responsive multidomain galectin from bay scallop Argopectens irradians[J]. FISH & SHELLFISH IMMUNOLOGY,2010,28(2):326-332.
APA	Song, Xiaoyan.,Zhang, Huan.,Zhao, Jianmin.,Wang, Lingling.,Qiu, Limei.,...&Song, Linsheng.(2010).An immune responsive multidomain galectin from bay scallop Argopectens irradians.FISH & SHELLFISH IMMUNOLOGY,28(2),326-332.
MLA	Song, Xiaoyan,et al."An immune responsive multidomain galectin from bay scallop Argopectens irradians".FISH & SHELLFISH IMMUNOLOGY 28.2(2010):326-332.

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