Foreign gene transfer into Chinese shrimps (Penaeus chinensis) with gene gun

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	Foreign gene transfer into Chinese shrimps (Penaeus chinensis) with gene gun
	Liu, ZY ; Xiang, JH; Zhou, GY ; Gong, ZX
	2001-05-01
发表期刊	CHINESE SCIENCE BULLETIN
ISSN	1001-6538
卷号	46 期号:9 页码:766-770
文章类型	Article
摘要	Plasmids pG DNA-RZ1 with a GFP (green fluorescent protein) reporter gene and a ribozyme gene incising penaeid white spot baculovirus (WSBV) were first introduced into the fertilized eggs of Chinese shrimps by gene gun. The treated and control samples of different development stages were observed with a fluorescent microscope. The transient expression of GFP gene was high in nauplius and zoea larvae. Results from RT-PCR and PCR for adults showed that the foreign genes had been transferred into the shrimps and had expressed the corresponding proteins. This work has established a transgenic method for penaeid shrimps, which will set base for the application of genetic engineering breeding into industry.; Plasmids pG DNA-RZ1 with a GFP (green fluorescent protein) reporter gene and a ribozyme gene incising penaeid white spot baculovirus (WSBV) were first introduced into the fertilized eggs of Chinese shrimps by gene gun. The treated and control samples of different development stages were observed with a fluorescent microscope. The transient expression of GFP gene was high in nauplius and zoea larvae. Results from RT-PCR and PCR for adults showed that the foreign genes had been transferred into the shrimps and had expressed the corresponding proteins. This work has established a transgenic method for penaeid shrimps, which will set base for the application of genetic engineering breeding into industry.
关键词	Gene Gun Gfp Transgenic Shrimps
学科领域	Multidisciplinary Sciences
收录类别	SCI
语种	英语
WOS记录号	WOS:000169054600017
引用统计	被引频次：8[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/3314
专题	实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Expt Marine Biol Lab, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Biochem, Shanghai 200031, Peoples R China
推荐引用方式 GB/T 7714	Liu, ZY,Xiang, JH,Zhou, GY,et al. Foreign gene transfer into Chinese shrimps (Penaeus chinensis) with gene gun[J]. CHINESE SCIENCE BULLETIN,2001,46(9):766-770.
APA	Liu, ZY,Xiang, JH,Zhou, GY,&Gong, ZX.(2001).Foreign gene transfer into Chinese shrimps (Penaeus chinensis) with gene gun.CHINESE SCIENCE BULLETIN,46(9),766-770.
MLA	Liu, ZY,et al."Foreign gene transfer into Chinese shrimps (Penaeus chinensis) with gene gun".CHINESE SCIENCE BULLETIN 46.9(2001):766-770.

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