Expression of TNF-alpha gene in Anabaena sp PCC 7120 and purification of its product by affinity chromatography

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	Expression of TNF-alpha gene in Anabaena sp PCC 7120 and purification of its product by affinity chromatography
	Shi, DJ ; Ye, X ; Zhong, H ; Cai, YY ; Shao, N ; Peng, GH ; Dong, XJ ; Wang, CM ; Ouyang, YX ; Li, ZJ
	2001
发表期刊	ACTA BOTANICA SINICA
ISSN	0577-7496
卷号	43 期号:1 页码:46-50
文章类型	Article
摘要	The effects of N (NaNO3) and C (NaAc) source in medium on the expression of tumor necrosis factor-alpha (TNF-alpha) gene in transgenic Anabaena sp. PCC 7120 were compared. The data showed that N source stabilized the expression of foreign protein and C source altered the synthesis of cell walls. Comparing several methods for breaking the cells, supersonic was able to extract TNF-alpha better than others. For purification of TNF-alpha, transgenic Anabaena cells were broken, the extracts were precipitated with ammonia sulfate, and the impure TNF-alpha was eluted from DEAE ion exchange chromatography. Electrophoresis (PAGE-SDS) showed a single band at 17 kD position.; The effects of N (NaNO3) and C (NaAc) source in medium on the expression of tumor necrosis factor-alpha (TNF-alpha) gene in transgenic Anabaena sp. PCC 7120 were compared. The data showed that N source stabilized the expression of foreign protein and C source altered the synthesis of cell walls. Comparing several methods for breaking the cells, supersonic was able to extract TNF-alpha better than others. For purification of TNF-alpha, transgenic Anabaena cells were broken, the extracts were precipitated with ammonia sulfate, and the impure TNF-alpha was eluted from DEAE ion exchange chromatography. Electrophoresis (PAGE-SDS) showed a single band at 17 kD position.
关键词	Tumor Necrosis Factor-alpha (Tnf-alpha) Transgenic Cyanobacterium (Anabaena Sp Pcc 7120) Affinity Chromatography
学科领域	Biochemistry & Molecular Biology ; Plant Sciences
收录类别	SCI
语种	英语
WOS记录号	WOS:000166865700009
引用统计
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/3318
专题	实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Beijing 100093, Peoples R China 2.Univ Sci & Technol China, Sch Life Sci, Hefei 230027, Peoples R China 3.Chinese Peoples Liberat Army, Hosp 301, Beijing 100853, Peoples R China 4.Chinese Acad Sci, Inst Oceanol, Qingdao 266003, Peoples R China
推荐引用方式 GB/T 7714	Shi, DJ,Ye, X,Zhong, H,et al. Expression of TNF-alpha gene in Anabaena sp PCC 7120 and purification of its product by affinity chromatography[J]. ACTA BOTANICA SINICA,2001,43(1):46-50.
APA	Shi, DJ.,Ye, X.,Zhong, H.,Cai, YY.,Shao, N.,...&Li, ZJ.(2001).Expression of TNF-alpha gene in Anabaena sp PCC 7120 and purification of its product by affinity chromatography.ACTA BOTANICA SINICA,43(1),46-50.
MLA	Shi, DJ,et al."Expression of TNF-alpha gene in Anabaena sp PCC 7120 and purification of its product by affinity chromatography".ACTA BOTANICA SINICA 43.1(2001):46-50.

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