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A new method of Laminaria japonica strain selection and sporeling raising by the use of gametophyte clones
Li, DP; Zhou, ZG; Liu, HH; Wu, CY
1999-04-01
发表期刊HYDROBIOLOGIA
ISSN0018-8158
卷号399页码:473-476
文章类型Article
摘要One-celled female and male gametophytes of three Laminaria japonica strains were isolated, cultured and gametophyte clones were formed. A technique combining strain selection with sporeling raising by the use of these female and male gametophyte clones was studied. Experiments on 9 different crossing combinations was conducted in November of 1997 in Qingdao, P. R. China. The main economic characteristics, frond length and fresh weight, of sporophytes of different crossing combinations were measured. F-1 sporophytes of No. 2 showed a higher fresh weight and longer length, therefore, No. 2 (Wh860 + x Lid) was selected as a good combination. Its parental female and male gametophyte clones are being mass cultured for sporeling production. By this method, the time needed for strain selection was shortened from 5-6 to 2 years. As compared with the routine method of sporeling raising by the collection of zoospores, the time of sporeling raising of this method decrease by 50%, and the production cost is also reduced by 50%. It is believed that this method will be labour and time saving and a more economic way for strain selection and sporeling raising in L. japonica cultivation industry.; One-celled female and male gametophytes of three Laminaria japonica strains were isolated, cultured and gametophyte clones were formed. A technique combining strain selection with sporeling raising by the use of these female and male gametophyte clones was studied. Experiments on 9 different crossing combinations was conducted in November of 1997 in Qingdao, P. R. China. The main economic characteristics, frond length and fresh weight, of sporophytes of different crossing combinations were measured. F-1 sporophytes of No. 2 showed a higher fresh weight and longer length, therefore, No. 2 (Wh860 + x Lid) was selected as a good combination. Its parental female and male gametophyte clones are being mass cultured for sporeling production. By this method, the time needed for strain selection was shortened from 5-6 to 2 years. As compared with the routine method of sporeling raising by the collection of zoospores, the time of sporeling raising of this method decrease by 50%, and the production cost is also reduced by 50%. It is believed that this method will be labour and time saving and a more economic way for strain selection and sporeling raising in L. japonica cultivation industry.
关键词Laminaria Japonica Gametophyte Clones Crossing Strain Selection Sporeling Raising
学科领域Marine & Freshwater Biology
收录类别SCI ; ISTP
语种英语
WOS记录号WOS:000082676900059
引用统计
被引频次:35[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/3354
专题实验海洋生物学重点实验室
作者单位Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
推荐引用方式
GB/T 7714
Li, DP,Zhou, ZG,Liu, HH,et al. A new method of Laminaria japonica strain selection and sporeling raising by the use of gametophyte clones[J]. HYDROBIOLOGIA,1999,399:473-476.
APA Li, DP,Zhou, ZG,Liu, HH,&Wu, CY.(1999).A new method of Laminaria japonica strain selection and sporeling raising by the use of gametophyte clones.HYDROBIOLOGIA,399,473-476.
MLA Li, DP,et al."A new method of Laminaria japonica strain selection and sporeling raising by the use of gametophyte clones".HYDROBIOLOGIA 399(1999):473-476.
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