| Comparative analysis of methods for concentrating venom from jellyfish Rhopilema esculentum Kishinouye | |
Li Cuiping1,2; Yu Huahua1 ; Feng Jinhua1,2; Chen Xiaolin1,2; Li Pengcheng1
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| 2009-02-01 | |
| 发表期刊 | CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
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| ISSN | 0254-4059 |
| 卷号 | 27期号:1页码:172-176 |
| 文章类型 | Article |
| 摘要 | In this study, several methods were compared for the efficiency to concentrate venom from the tentacles of jellyfish Rhopilema esculentum Kishinouye. The results show that the methods using either freezing-dry or gel absorption to remove water to concentrate venom are not applicable due to the low concentration of the compounds dissolved. Although the recovery efficiency and the total venom obtained using the dialysis dehydration method are high, some proteins can be lost during the concentrating process. Comparing to the lyophilization method, ultrafiltration is a simple way to concentrate the compounds at high percentage but the hemolytic activities of the proteins obtained by ultrafiltration appear to be lower. Our results suggest that overall lyophilization is the best and recommended method to concentrate venom from the tentacles of jellyfish. It shows not only the high recovery efficiency for the venoms but high hemolytic activities as well.; In this study, several methods were compared for the efficiency to concentrate venom from the tentacles of jellyfish Rhopilema esculentum Kishinouye. The results show that the methods using either freezing-dry or gel absorption to remove water to concentrate venom are not applicable due to the low concentration of the compounds dissolved. Although the recovery efficiency and the total venom obtained using the dialysis dehydration method are high, some proteins can be lost during the concentrating process. Comparing to the lyophilization method, ultrafiltration is a simple way to concentrate the compounds at high percentage but the hemolytic activities of the proteins obtained by ultrafiltration appear to be lower. Our results suggest that overall lyophilization is the best and recommended method to concentrate venom from the tentacles of jellyfish. It shows not only the high recovery efficiency for the venoms but high hemolytic activities as well. |
| 关键词 | Concentration Hemolytic Activity Rhopilema Esculentum Kishinouye Venom |
| 学科领域 | Limnology ; Oceanography |
| DOI | 10.1007/s00343-009-0172-2 |
| URL | 查看原文 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000264616100027 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/3472 |
| 专题 | 海洋生物技术研发中心 海洋环境工程技术研究发展中心 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 第一作者单位 | 中国科学院海洋研究所 |
| 推荐引用方式 GB/T 7714 | Li Cuiping,Yu Huahua,Feng Jinhua,et al. Comparative analysis of methods for concentrating venom from jellyfish Rhopilema esculentum Kishinouye[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2009,27(1):172-176. |
| APA | Li Cuiping,Yu Huahua,Feng Jinhua,Chen Xiaolin,&Li Pengcheng.(2009).Comparative analysis of methods for concentrating venom from jellyfish Rhopilema esculentum Kishinouye.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,27(1),172-176. |
| MLA | Li Cuiping,et al."Comparative analysis of methods for concentrating venom from jellyfish Rhopilema esculentum Kishinouye".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 27.1(2009):172-176. |
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