Study on early-stage development of conchospore in Porphyra yezoensis Ueda

doi:10.1016/j.aquaculture.2008.02.037

IOCAS-IR > 实验海洋生物学重点实验室

	Study on early-stage development of conchospore in Porphyra yezoensis Ueda
	Fan Xiaolei 1,2; Wang Guangce 1,3; Li Demao 1,2; Xu Pu 4; Shen Songdong 5
	2008-06-10
发表期刊	AQUACULTURE
ISSN	0044-8486
卷号	278 期号:1-4 页码:143-149
文章类型	Article
摘要	Porphyra yezoensis Ueda (Rhodophyta) is a seaweed of economic importance with a typical dimorphic life cycle consisting of a leafy gametophyte and a filamentous sporophyte. Recently, it has been recognized as a model system for fundamental and applied studies in marine biological sciences. Conchospore, a major spore linking the two distinct multicellular phases in the life cycle, is most widely used in the breeding of P. yezoensis. In this paper, the early-stage development of conchospore, including the attachment and the cell wall formation, was studied with fluorescent reagents staining and Scanning Electron Microscopy detection. Results displayed: (I) the cell wall began to be generated after culturing for 4 h in the attached conchospores; (2) the initially released conchospores were plastids with some filmy, amorphous substance on the surface, and they attached to the fibers firmly via the actively secreted mucilaginous substances after their touch to the fibers; (3) cellulase and pectolase prohibited the attachment of conchospores in the different ways; and (4) only attached conchospores generated cell walls and developed normally, while the suspending ones could not. It indicated that the cellulose played crucial roles in the permanent attachment as the pectin did in the initial attachment. The conchospore attachment seemed to trigger the cell wall formation and the further development. Affects of light on the development of conchospores were also discussed. The results showed that high intensity (200 mu mol.m(-2).s(-1)) and long-wave (>= 580 nm) light facilitated the division rate of conchospores. (C) 2008 Elsevier B.V. All rights reserved.; Porphyra yezoensis Ueda (Rhodophyta) is a seaweed of economic importance with a typical dimorphic life cycle consisting of a leafy gametophyte and a filamentous sporophyte. Recently, it has been recognized as a model system for fundamental and applied studies in marine biological sciences. Conchospore, a major spore linking the two distinct multicellular phases in the life cycle, is most widely used in the breeding of P. yezoensis. In this paper, the early-stage development of conchospore, including the attachment and the cell wall formation, was studied with fluorescent reagents staining and Scanning Electron Microscopy detection. Results displayed: (I) the cell wall began to be generated after culturing for 4 h in the attached conchospores; (2) the initially released conchospores were plastids with some filmy, amorphous substance on the surface, and they attached to the fibers firmly via the actively secreted mucilaginous substances after their touch to the fibers; (3) cellulase and pectolase prohibited the attachment of conchospores in the different ways; and (4) only attached conchospores generated cell walls and developed normally, while the suspending ones could not. It indicated that the cellulose played crucial roles in the permanent attachment as the pectin did in the initial attachment. The conchospore attachment seemed to trigger the cell wall formation and the further development. Affects of light on the development of conchospores were also discussed. The results showed that high intensity (200 mu mol.m(-2).s(-1)) and long-wave (>= 580 nm) light facilitated the division rate of conchospores. (C) 2008 Elsevier B.V. All rights reserved.
关键词	Attachment Cell Wall Formation Conchospore Light Effects Porphyra Yezoensis
学科领域	Fisheries ; Marine & Freshwater Biology
DOI	10.1016/j.aquaculture.2008.02.037
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000257004800021
引用统计	被引频次：16[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/5540
专题	实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 3.Tianjin Univ Sci & Technol, Coll Marine Sci & Engn, Tianjin 300457, Peoples R China 4.Changshu Inst Technol, Changshu 215500, Peoples R China 5.Soochow Univ, Sch Life Sci, Suzhou 215006, Peoples R China
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Fan Xiaolei,Wang Guangce,Li Demao,et al. Study on early-stage development of conchospore in Porphyra yezoensis Ueda[J]. AQUACULTURE,2008,278(1-4):143-149.
APA	Fan Xiaolei,Wang Guangce,Li Demao,Xu Pu,&Shen Songdong.(2008).Study on early-stage development of conchospore in Porphyra yezoensis Ueda.AQUACULTURE,278(1-4),143-149.
MLA	Fan Xiaolei,et al."Study on early-stage development of conchospore in Porphyra yezoensis Ueda".AQUACULTURE 278.1-4(2008):143-149.

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