Induction and characterization of green pigmentation mutant in Porphyra yezoensis Ueda

doi:10.1016/j.aquaculture.2008.05.026

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	Induction and characterization of green pigmentation mutant in Porphyra yezoensis Ueda
	Li, Ying Xia 1,2; Wang, Guang Ce 1,3; Xu, Pu 4; Fan, Xiao Lei 1,2; Niu, Jian Feng 1,2; Zhou, Bai Cheng 1
	2008-09-30
发表期刊	AQUACULTURE
ISSN	0044-8486
卷号	282 期号:1-4 页码:117-123
文章类型	Article
摘要	The free living conchocelis of Porphyra yezoensis Ueda was treated with N-methyl-N-nitro-N-nitrosoguanidine to induce pigmentation mutants. The artificial green pigmentation mutant of P. yezoensis conchocelis, which was composed entirely of green cells, was isolated through visualization with the unaided eye. The acquired green conchocelis was further developed into a green gametophytic blade. This mutant was relatively stable in color in both gametophytic blade and conchocelis phases. The gametophytic blade mutant was successively cultivated for commerce at some Porphyra farms in Rudong, China, and few wild type or sectorially variegated gametophytic blade occurred, indicating that the green mutant has commercial value. The green mutant was characterized as having lower phycoerythrin and higher phycocyanin content, and SDS-PAGE suggested that phycoerythrin was missing the gamma-subunit in comparison to the wild type. The wild type and the green mutant showed a clear difference in 02 evolution rates in white, green, yellow, and red light, which might be due to the qualitative and quantitative changes of phycoerythrin, and the quantitative difference of phycocyanin. (C) 2008 Elsevier B.V. All rights reserved.; The free living conchocelis of Porphyra yezoensis Ueda was treated with N-methyl-N-nitro-N-nitrosoguanidine to induce pigmentation mutants. The artificial green pigmentation mutant of P. yezoensis conchocelis, which was composed entirely of green cells, was isolated through visualization with the unaided eye. The acquired green conchocelis was further developed into a green gametophytic blade. This mutant was relatively stable in color in both gametophytic blade and conchocelis phases. The gametophytic blade mutant was successively cultivated for commerce at some Porphyra farms in Rudong, China, and few wild type or sectorially variegated gametophytic blade occurred, indicating that the green mutant has commercial value. The green mutant was characterized as having lower phycoerythrin and higher phycocyanin content, and SDS-PAGE suggested that phycoerythrin was missing the gamma-subunit in comparison to the wild type. The wild type and the green mutant showed a clear difference in 02 evolution rates in white, green, yellow, and red light, which might be due to the qualitative and quantitative changes of phycoerythrin, and the quantitative difference of phycocyanin. (C) 2008 Elsevier B.V. All rights reserved.
关键词	Porphyra Yezoensis Ueda The Green Pigmentation Mutant Wild Type Conchocelis Gametophytic Blade
学科领域	Fisheries ; Marine & Freshwater Biology
DOI	10.1016/j.aquaculture.2008.05.026
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000260292000018
引用统计	被引频次：14[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/5586
专题	实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 3.Tianjin Univ Sci & Technol, Coll Marine Sci & Engn, Tianjin 300457, Peoples R China 4.Changshu Inst Technol, Dept Biol, Changshu 215500, Peoples R China
第一作者单位	实验海洋生物学重点实验室
推荐引用方式 GB/T 7714	Li, Ying Xia,Wang, Guang Ce,Xu, Pu,et al. Induction and characterization of green pigmentation mutant in Porphyra yezoensis Ueda[J]. AQUACULTURE,2008,282(1-4):117-123.
APA	Li, Ying Xia,Wang, Guang Ce,Xu, Pu,Fan, Xiao Lei,Niu, Jian Feng,&Zhou, Bai Cheng.(2008).Induction and characterization of green pigmentation mutant in Porphyra yezoensis Ueda.AQUACULTURE,282(1-4),117-123.
MLA	Li, Ying Xia,et al."Induction and characterization of green pigmentation mutant in Porphyra yezoensis Ueda".AQUACULTURE 282.1-4(2008):117-123.

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