Comparison of Gene Expression Profiles of Fenneropenaeus chinensis Challenged with WSSV and Vibrio

doi:10.1007/s10126-008-9105-x

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	Comparison of Gene Expression Profiles of Fenneropenaeus chinensis Challenged with WSSV and Vibrio
	Wang, Bing 1; Li, Fuhua 1; Luan, Wei 1,2; Xie, Yusu 1,2; Zhang, Chengsong1,2 ; Luo, Zhan 1,2; Gui, Lang 1,2; Yan, Hui 1,2; Xiang, Jianhai1
	2008-12-01
发表期刊	MARINE BIOTECHNOLOGY
ISSN	1436-2228
卷号	10 期号:6 页码:664-675
文章类型	Article
摘要	Microarray technique was used to analyze the gene expression profiles of shrimp when they were challenged by WSSV and heat-inactivated Vibrio anguillarum, respectively. At 6 h post challenge (HPC), a total of 806 clones showed differential expression profile in WSSV-challenged samples, but not in Vibrio-challenged samples. The genes coding energy metabolism enzyme and structure protein were the most downregulated elements in 6 h post WSSV-challenged (HPC-WSSV) tissues. However, a total of 155 clones showed differential expression in the Vibrio-challenged samples, but not in WSSV-challenged samples. Serine-type endopeptidase and lysosome-related genes were the most upregulated elements in tissues 6 h post Vibrio challenge (HPC-Vibrio). Totally, 188 clones showed differential expression in both 6 and 12 HPC-WSSV and HPC-Vibrio samples. Most of the differentially expressed genes (185/188) were downregulated in the samples of 12 HPC-WSSV, whereas upregulated in the samples at 6 and 12 HPC-Vibrio and 6 HPC-WSSV. The expression profiles of three differentially expressed genes identified in microarray hybridization were analyzed in hemocytes, lymphoid organ, and hepatopancreas of shrimp challenged by WSSV or Vibrio through real-time PCR. The results further confirmed the microarray hybridization results. The data will provide great help for us in understanding the immune mechanism of shrimp responding to WSSV or Vibrio.; Microarray technique was used to analyze the gene expression profiles of shrimp when they were challenged by WSSV and heat-inactivated Vibrio anguillarum, respectively. At 6 h post challenge (HPC), a total of 806 clones showed differential expression profile in WSSV-challenged samples, but not in Vibrio-challenged samples. The genes coding energy metabolism enzyme and structure protein were the most downregulated elements in 6 h post WSSV-challenged (HPC-WSSV) tissues. However, a total of 155 clones showed differential expression in the Vibrio-challenged samples, but not in WSSV-challenged samples. Serine-type endopeptidase and lysosome-related genes were the most upregulated elements in tissues 6 h post Vibrio challenge (HPC-Vibrio). Totally, 188 clones showed differential expression in both 6 and 12 HPC-WSSV and HPC-Vibrio samples. Most of the differentially expressed genes (185/188) were downregulated in the samples of 12 HPC-WSSV, whereas upregulated in the samples at 6 and 12 HPC-Vibrio and 6 HPC-WSSV. The expression profiles of three differentially expressed genes identified in microarray hybridization were analyzed in hemocytes, lymphoid organ, and hepatopancreas of shrimp challenged by WSSV or Vibrio through real-time PCR. The results further confirmed the microarray hybridization results. The data will provide great help for us in understanding the immune mechanism of shrimp responding to WSSV or Vibrio.
关键词	Wssv Vibrio Cdna Microarray Fenneropenaeus Chinensis
学科领域	Biotechnology & Applied Microbiology ; Marine & Freshwater Biology
DOI	10.1007/s10126-008-9105-x
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000260838600005
引用统计	被引频次：46[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/5660
专题	实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Wang, Bing,Li, Fuhua,Luan, Wei,et al. Comparison of Gene Expression Profiles of Fenneropenaeus chinensis Challenged with WSSV and Vibrio[J]. MARINE BIOTECHNOLOGY,2008,10(6):664-675.
APA	Wang, Bing.,Li, Fuhua.,Luan, Wei.,Xie, Yusu.,Zhang, Chengsong.,...&Xiang, Jianhai.(2008).Comparison of Gene Expression Profiles of Fenneropenaeus chinensis Challenged with WSSV and Vibrio.MARINE BIOTECHNOLOGY,10(6),664-675.
MLA	Wang, Bing,et al."Comparison of Gene Expression Profiles of Fenneropenaeus chinensis Challenged with WSSV and Vibrio".MARINE BIOTECHNOLOGY 10.6(2008):664-675.

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