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Cloning and recombinant expression of a crustin-like gene from Chinese shrimp, Fenneropenaeus chinensis
Zhang, Jiquan; Li, Fuhua; Wang, Zaizhao; Xiang, Jianhai
2007-01-20
发表期刊JOURNAL OF BIOTECHNOLOGY
ISSN0168-1656
卷号127期号:4页码:605-614
文章类型Article
摘要Antimicrobial peptides or proteins (AMPs) are proved to be one of the most important humoral factors to resist pathogen infection. As an antimicrobial protein, crustin had been described in invertebrates as a component of the innate immune system. A crustin-like gene (CruFc) was cloned from haemocytes of Chinese shrimp Fenneropenaeus chinensis by 3' and 5'-RACE PCR. The full-length cDNA consists of 523 with 405 bp open reading frame encoding 134 amino acids and the deduced peptide contains a putative signal peptide of 17 amino acids. The sequence also contains a whey-acidic protein (WAP) domain at the C-terminal. Transcripts of CruFc were mainly detected in haemocytes and gill by RT-PCR analysis. In addition, another full-length cDNA named CshFc was also cloned from haemocytes of Chinese shrimp and its inferred amino acid sequence lacks the WAP-type 'four-disulfide core' domain. The fusion proteins containing CruFc and CshFc were, respectively, produced and the antimicrobial assays revealed that the recombinant CruFc could inhibit the growth of grain-positive bacteria in vitro but the recombinant CshFc could not inhibit at the same conditions. The difference of antimicrobial activity between recombinant CruFc and CshFc provides the evidence that the four-disulfide core domain of crustin may play an important role in its biological function. (c) 2006 Elsevier B.V. All rights reserved.; Antimicrobial peptides or proteins (AMPs) are proved to be one of the most important humoral factors to resist pathogen infection. As an antimicrobial protein, crustin had been described in invertebrates as a component of the innate immune system. A crustin-like gene (CruFc) was cloned from haemocytes of Chinese shrimp Fenneropenaeus chinensis by 3' and 5'-RACE PCR. The full-length cDNA consists of 523 with 405 bp open reading frame encoding 134 amino acids and the deduced peptide contains a putative signal peptide of 17 amino acids. The sequence also contains a whey-acidic protein (WAP) domain at the C-terminal. Transcripts of CruFc were mainly detected in haemocytes and gill by RT-PCR analysis. In addition, another full-length cDNA named CshFc was also cloned from haemocytes of Chinese shrimp and its inferred amino acid sequence lacks the WAP-type 'four-disulfide core' domain. The fusion proteins containing CruFc and CshFc were, respectively, produced and the antimicrobial assays revealed that the recombinant CruFc could inhibit the growth of grain-positive bacteria in vitro but the recombinant CshFc could not inhibit at the same conditions. The difference of antimicrobial activity between recombinant CruFc and CshFc provides the evidence that the four-disulfide core domain of crustin may play an important role in its biological function. (c) 2006 Elsevier B.V. All rights reserved.
关键词Fenneropenaeus Chinensis Crustin-like Gene Prokaryotic Expression Function Identification
学科领域Biotechnology & Applied Microbiology
DOI10.1016/j.jbiotec.2006.08.013
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收录类别SCI
语种英语
WOS记录号WOS:000243704600005
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被引频次:112[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/5724
专题实验海洋生物学重点实验室
作者单位1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
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Zhang, Jiquan,Li, Fuhua,Wang, Zaizhao,et al. Cloning and recombinant expression of a crustin-like gene from Chinese shrimp, Fenneropenaeus chinensis[J]. JOURNAL OF BIOTECHNOLOGY,2007,127(4):605-614.
APA Zhang, Jiquan,Li, Fuhua,Wang, Zaizhao,&Xiang, Jianhai.(2007).Cloning and recombinant expression of a crustin-like gene from Chinese shrimp, Fenneropenaeus chinensis.JOURNAL OF BIOTECHNOLOGY,127(4),605-614.
MLA Zhang, Jiquan,et al."Cloning and recombinant expression of a crustin-like gene from Chinese shrimp, Fenneropenaeus chinensis".JOURNAL OF BIOTECHNOLOGY 127.4(2007):605-614.
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