Antioxidant properties of recombinant allophycocyanin expressed in Escherichia coli

doi:10.1016/j.jphotobiol.2006.02.008

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	Antioxidant properties of recombinant allophycocyanin expressed in Escherichia coli
	Ge, Baosheng ; Qin, Song ; Han, Lu ; Lin, Fan ; Ren, Yuhong
	2006-09-01
发表期刊	JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY
ISSN	1011-1344
卷号	84 期号:3 页码:175-180
文章类型	Article
摘要	Allophycocyanin (A-PC) is the main core component of phycobilisome found in blue-green algae. The apo-allophycocyanin and its subunits were expressed in Escherichia coli and their antioxidant properties were evaluated using deoxyribose assay. The result showed that both recombinant allophycocyanin fused with maltose binding protein (MBP) tag and 6 x His-tag and their alpha or beta subunits can scavenge hydroxyl radicals successfully, and the separated g or beta subunits had a higher inhibition effect on hydroxyl radicals than that when they combined together. The scavenging effects increased with the increasing concentration. These results clearly suggested that apo-allophycocyanin is involved in the antioxidant and radical scavenging activity of phycocyanin, and the antioxidant activity may be partially responsible to the anti-tumor effect of the recombinant allophycocyanin. (c) 2006 Elsevier B.V. All rights reserved.; Allophycocyanin (A-PC) is the main core component of phycobilisome found in blue-green algae. The apo-allophycocyanin and its subunits were expressed in Escherichia coli and their antioxidant properties were evaluated using deoxyribose assay. The result showed that both recombinant allophycocyanin fused with maltose binding protein (MBP) tag and 6 x His-tag and their alpha or beta subunits can scavenge hydroxyl radicals successfully, and the separated g or beta subunits had a higher inhibition effect on hydroxyl radicals than that when they combined together. The scavenging effects increased with the increasing concentration. These results clearly suggested that apo-allophycocyanin is involved in the antioxidant and radical scavenging activity of phycocyanin, and the antioxidant activity may be partially responsible to the anti-tumor effect of the recombinant allophycocyanin. (c) 2006 Elsevier B.V. All rights reserved.
关键词	Antioxidant Recombinant Allophycocyanin Escherichia Coli Anti-tumor Hydroxyl Radicals
学科领域	Biochemistry & Molecular Biology ; Biophysics
DOI	10.1016/j.jphotobiol.2006.02.008
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000240212600002
引用统计	被引频次：44[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/5832
专题	实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Grad Univ Chinese Acad Sci, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Ge, Baosheng,Qin, Song,Han, Lu,et al. Antioxidant properties of recombinant allophycocyanin expressed in Escherichia coli[J]. JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY,2006,84(3):175-180.
APA	Ge, Baosheng,Qin, Song,Han, Lu,Lin, Fan,&Ren, Yuhong.(2006).Antioxidant properties of recombinant allophycocyanin expressed in Escherichia coli.JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY,84(3),175-180.
MLA	Ge, Baosheng,et al."Antioxidant properties of recombinant allophycocyanin expressed in Escherichia coli".JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY 84.3(2006):175-180.

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